Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii
| Autor: | Jean-Marie Lacroix, Edwige Madec, Sébastien Bontemps-Gallo |
|---|---|
| Přispěvatelé: | Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 (UGSF), Institut National de la Recherche Agronomique (INRA)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), CNRS, Université de Lille, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF], Université de Lille-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
musculoskeletal diseases
Hydrolases Mutant Virulence Repressor Microbiology Gene Knockout Techniques Osmoregulation Bacterial Proteins Enterobacteriaceae Secretion PecS Gene Glucans Osmoregulated periplasmic glucans biology regulation Periplasmic space 15. Life on land biology.organism_classification Dickeya dadantii [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology plant pathogen Repressor Proteins D. dadantii Bacteria Locomotion |
| Zdroj: | Microbiology Microbiology, Microbiology Society, 2014, 160 (4), pp.766-777. ⟨10.1099/mic.0.074484-0⟩ Microbiology, 2014, 160 (4), pp.766-777. ⟨10.1099/mic.0.074484-0⟩ |
| ISSN: | 1350-0872 1465-2080 |
| DOI: | 10.1099/mic.0.074484-0⟩ |
| Popis: | Dickeya dadantii is a phytopathogenic enterobacterium that causes soft rot disease in a wide range of plant species. Maceration, an apparent symptom of the disease, is the result of the synthesis and secretion of a set of plant cell wall-degrading enzymes (PCWDEs), but many additional factors are required for full virulence. Among these, osmoregulated periplasmic glucans (OPGs) and the PecS transcriptional regulator are essential virulence factors. Several cellular functions are controlled by both OPGs and PecS. Strains devoid of OPGs display a pleiotropic phenotype including total loss of virulence, loss of motility and severe reduction in the synthesis of PCWDEs. PecS is one of the major regulators of virulence in D. dadantii, acting mainly as a repressor of various cellular functions including virulence, motility and synthesis of PCWDEs. The present study shows that inactivation of the pecS gene restored virulence in a D. dadantii strain devoid of OPGs, indicating that PecS cannot be de-repressed in strains devoid of OPGs. |
| Databáze: | OpenAIRE |
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