Developmental regulation and tissue-specific differences of heat shock gene expression in transgenic tobacco and Arabidopsis plants
| Autor: | Eva Kloske, Ralf Prändl, Fritz Schöffl |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Arabidopsis
Plant Science Species Specificity Gene Expression Regulation Plant Genes Reporter Heat shock protein Tobacco Gene expression Genetics Tissue Distribution Promoter Regions Genetic Heat-Shock Proteins In Situ Hybridization Vascular tissue Glucuronidase Regulation of gene expression Reporter gene biology Developmental induction fungi food and beverages General Medicine Meristem Plants Genetically Modified biology.organism_classification Molecular biology Plant Leaves Plants Toxic Soybeans Agronomy and Crop Science |
| Zdroj: | Plant Molecular Biology. 28:73-82 |
| ISSN: | 1573-5028 0167-4412 |
| DOI: | 10.1007/bf00042039 |
| Popis: | The heat shock (hs) response during plant growth and development was analyzed in tobacco and Arabidopsis using chimaeric beta-glucuronidase reporter genes (hs-Gus) driven by a soybean hs promoter. Fluorimetric measurements and histochemical staining revealed high Gus activities in leaves, roots, and flowers exclusively after heat stress. The highest levels of heat-inducible expression were found in the vascular tissues. Without heat stress, a developmental induction of hs-Gus was indicated by the accumulation of high levels of Gus in transgenic tobacco seeds. There was no developmental induction of hs-Gus in Arabidopsis seeds. In situ hybridization to the RNA of the small heat shock protein gene Athsp17.6 in tissue sections revealed an expression in heat-shocked leaves but no expression in control leaves of Arabidopsis. However, a high level of constitutive expression of hs genes was detected in meristematic and provascular tissues of the Arabidopsis embryo. The developmental and tissue-specific regulation of the hs response is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink