Biological effects of ubiquitin-specific peptidase 22 on thyroid papillary cancer cells and its mechanism of action
| Autor: | Huaiyin Shi, Ying-Xue Li, Hong-Qun Wang, Shan-Shan Ding, Ying Li, Ai-Chun Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
biology Thyroid papillary cancer Chemistry cyclin D2 Papillary thyroid cancer ubiquitin-specific peptidase 22 (USP22) Oncology Mechanism of action Ubiquitin thyroid papillary cancer-1 (TPC-1) medicine biology.protein Cancer research Original Article Radiology Nuclear Medicine and imaging Bmi-1 medicine.symptom |
| Zdroj: | Translational Cancer Research |
| ISSN: | 2219-6803 2218-676X |
| DOI: | 10.21037/tcr-20-2102 |
| Popis: | Background In this study, ubiquitin-specific peptidase 22 (USP22) was detected in both thyroid papillary cancer-1 (TPC-1) and normal thyroid epithelial cell lines (HT-ori3), and its biological function was analyzed. Method Cell culture, resuscitation, and passage, Western blot, and real-time polymerase chain reaction were used. Results The expression of USP22 was found to be significantly higher in TPC-1 cancer cells than in normal cells. After silencing of the USP22 gene in TPC-1 cells, the levels of USP22 gene and protein expression were significantly decreased. After 6 h with silencing of the USP22 gene, the migration rate was lower and the cells had become smaller than in the control group (P |
| Databáze: | OpenAIRE |
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