Measuring surface potential changes on leaves
| Autor: | Seyed Ali Reza Mousavi, Edward E. Farmer, Stephan Kellenberger, Chi Tam Nguyen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
0106 biological sciences
Surface Properties Mrna expression Arabidopsis Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Membrane Potentials 03 medical and health sciences Botany Arabidopsis thaliana Arabidopsis/physiology Electrodes Ion channel 030304 developmental biology 0303 health sciences fungi Plant physiology food and beverages biology.organism_classification Electrophysiology/methods Electrophysiology Plant Leaves Biophysics Plant Leaves/physiology 010606 plant biology & botany Signal Transduction |
| Zdroj: | Nature protocols Nature Protocols |
| DOI: | 10.1038/nprot.2014.136 |
| Popis: | We provide here a detailed protocol for studying the changes in electrical surface potential of leaves. This method has been developed over the years by plant physiologists and is currently used in different variants in many laboratories. The protocol records surface potential changes to measure long distance electrical signals induced by diverse stimuli such as leaf wounding or current injection. This technique can be used to determine signaling speeds to measure the connectivity between different plant organs and by exploiting mutant plants to identify transporters and ion channels involved in electrical signaling. The approach can be combined with the analysis of mRNA expression and of metabolite concentrations to correlate electrical signaling to specific physiological events. We describe how to use this protocol on Arabidopsis looking at the effects of leaf wounding; however it is broadly applicable to other plants and can be used to study other aspects of plant physiology. After wound infliction surface potential recording takes approximately 20 min per plant. |
| Databáze: | OpenAIRE |
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