Impairment of Interleukin-17A Expression in Canine Visceral Leishmaniosis is Correlated with Reduced Interferon-γ and Inducible Nitric Oxide Synthase Expression
| Autor: | M. T. Bahia, Maria Adelaide Do-Valle-Matta, Ivo Santana Caldas, Janeusa Trindade de Souto, Manuela Sales Lima Nascimento, Paulo Marcos da Matta Guedes, Álvaro Fernando da Silva do Nascimento, Antônia Cláudia Jácome da Câmara, T.D.R. Albuquerque, Lúcia Maria da Cunha Galvão, André Talvani |
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| Rok vydání: | 2015 |
| Předmět: |
Nitric Oxide Synthase Type II
Spleen Enzyme-Linked Immunosorbent Assay Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Interferon-gamma Immune system Dogs Interferon medicine Animals Interferon gamma Dog Diseases General Veterinary biology Interleukin-17 Interleukin biology.organism_classification Nitric oxide synthase medicine.anatomical_structure Immunology biology.protein Cytokines Leishmaniasis Visceral Interleukin 17 Leishmania infantum medicine.drug |
| Zdroj: | Journal of comparative pathology. 153(4) |
| ISSN: | 1532-3129 |
| Popis: | Dogs are the primary urban reservoir of Leishmania infantum and play a crucial role in the transmission of this parasite to man via sandflies. The spleen and liver are the main target organs of L. infantum infection, but few studies have evaluated the immune response to this infection in the canine liver. To identify the immunological mediators involved in resistance and/or susceptibility to canine visceral leishmaniosis (CVL), we selected 21 dogs naturally infected by L. infantum and classified as asymptomatic or symptomatic. Immunological parameters were analysed and correlations with clinical signs were determined. Symptomatic dogs showed higher numbers of parasites and less leucocyte infiltration in the liver compared with asymptomatic dogs. The progression of this disease was characterized not only by the down regulation of T helper (Th) 1-related cytokines, such as interferon (IFN)-γ and tumour necrosis factor (TNF)-α, but also by the down regulation of genes encoding interleukin (IL)-17A, inducible nitric oxide synthase (iNOS) and IL-10 in the spleen and liver in symptomatic dogs compared with asymptomatic dogs. Importantly, IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-γ. Th1- and Th17-related cytokines therefore appear to play a role in restricting parasite growth via iNOS activation and decrease susceptibility of dogs to CVL. |
| Databáze: | OpenAIRE |
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