Activation of Enteroendocrine Cells via TLRs Induces Hormone, Chemokine, and Defensin Secretion
| Autor: | Marco Palazzo, Graziano Serrao, Claudia Calcaterra, Silvia Selleri, Anna Rossini, Silvia Gariboldi, Y.F. Shirai, Laura Zanobbio, Andrea Balsari, Cristiano Rumio, Francesca Arnaboldi |
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| Rok vydání: | 2007 |
| Předmět: |
Lipopolysaccharides
Chemokine medicine.medical_specialty beta-Defensins Enteroendocrine Cells Immunology Enteroendocrine cell Stimulation Biology Cell Line Mice Internal medicine medicine Animals Humans Immunology and Allergy RNA Small Interfering Receptor Defensin Protein Kinase C Cholecystokinin Toll-Like Receptors Cell biology Mice Inbred C57BL Endocrinology Oligodeoxyribonucleotides Myeloid Differentiation Factor 88 biology.protein TLR4 Female Chemokines Flagellin |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.7.4296 |
| Popis: | Enteroendocrine cells are known primarily for their production of hormones that affect digestion, but they might also be implicated in sensing and neutralizing or expelling pathogens. We evaluate the expression of TLRs and the response to specific agonists in terms of cytokines, defensins, and hormones in enteroendocrine cells. The mouse enteroendocrine cell line STC-1 and C57BL/6 mice are used for in vitro and in vivo studies, respectively. The presence of TLR4, 5, and 9 is investigated by RT-PCR, Western blot, and immunofluorescence analyses. Activation of these receptors is studied evaluating keratinocyte-derived chemokine, defensins, and cholecystokinin production in response to their specific agonists. In this study, we show that the intestinal enteroendocrine cell line STC-1 expresses TLR4, 5, and 9 and releases cholecystokinin upon stimulation with the respective receptor agonists LPS, flagellin, and CpG-containing oligodeoxynucleotides. Release of keratinocyte-derived chemokine and β-defensin 2 was also observed after stimulation of STC-1 cells with the three TLR agonists, but not with fatty acids. Consistent with these in vitro data, mice showed increased serum cholecystokinin levels after oral challenge with LPS, flagellin, or CpG oligodeoxynucleotides. In addition to their response to food stimuli, enteroendocrine cells sense the presence of bacterial Ags through TLRs and are involved in neutralizing intestinal bacteria by releasing chemokines and defensins, and maybe in removing them by releasing hormones such as cholecystokinin, which induces contraction of the muscular tunica, favoring the emptying of the distal small intestine. |
| Databáze: | OpenAIRE |
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