Inoculation and co-inoculation of two monosporic fungi onto surface-sterile blackberry fruits for quantification experiments
| Autor: | Ramón Molina-Bravo, Irena Hilje-Rodríguez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Clinical Biochemistry
Biological pest control Fungus 010501 environmental sciences 01 natural sciences Rubus adenotrichos 03 medical and health sciences Quantitative PCR Fungal Structures lcsh:Science 030304 developmental biology 0105 earth and related environmental sciences Botrytis cinerea 0303 health sciences biology Inoculation Phytopathology food and beverages Method Article biology.organism_classification Medical Laboratory Technology Horticulture Trichoderma Biological control Co-inoculation of fungi onto surface-sterile blackberry fruits lcsh:Q Antagonism Co inoculation |
| Zdroj: | MethodsX MethodsX, Vol 7, Iss, Pp 101092-(2020) |
| ISSN: | 2215-0161 |
| Popis: | Trichoderma is used as a biocontrol agent against different plant pathogens in different crops. In Costa Rica, Trichoderma isolates from blackberry fruits (Rubus adenotrichos Schltdl.) have shown antagonism in laboratory and field trials against Botrytis cinerea. Quantifying fungal antagonistic activity directly on target organs or target tissues is of interest to estimate the performance of biocontrol agents. However, this is difficult due to the lack of visual manifestations of fungal structures. As part of a larger study to quantify antagonistic activity by quantitative PCR, we detail here a method to isolate and purify each fungus and then inoculate or co-inoculate them onto surface-sterilized blackberry fruits. • A procedure to co-inoculate the surfaces of blackberry fruits with monosporic fungal suspensions for molecular analyses is described. • The protocol described herein was implemented for subsequent qPCR analysis. Graphical abstract Image, graphical abstract |
| Databáze: | OpenAIRE |
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