Quantifying ATP turnover in anoxic coleoptiles of rice (Oryza sativa) demonstrates preferential allocation of energy to protein synthesis
| Autor: | Brian J. Atwell, Thomas H. Roberts, Joshua M. Edwards |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Plant Bioenergetics Physiology Cellular respiration Cell Respiration Germination Oryza sativa Plant Science Biology ATP utilization Cell wall chemistry.chemical_compound Adenosine Triphosphate Anoxia Cell Wall Protein biosynthesis Plant Proteins chemistry.chemical_classification hypoxia rice Cell Membrane food and beverages Biological Transport Oryza Lipids Oxygen Coleoptile Enzyme Biochemistry chemistry RNA Plant Seedlings Protein Biosynthesis Fermentation Mutation Energy Metabolism Cotyledon Adenosine triphosphate Research Paper |
| Zdroj: | Journal of Experimental Botany |
| ISSN: | 1460-2431 0022-0957 |
| Popis: | Oxygen deprivation limits the energy available for cellular processes and yet no comprehensive ATP budget has been reported for any plant species under O(2) deprivation, including Oryza sativa. Using 3-d-old coleoptiles of a cultivar of O. sativa tolerant to flooding at germination, (i) rates of ATP regeneration in coleoptiles grown under normoxia (aerated solution), hypoxia (3% O(2)), and anoxia (N(2)) and (ii) rates of synthesis of proteins, lipids, nucleic acids, and cell walls, as well as K(+) transport, were determined. Based on published bioenergetics data, the cost of synthesizing each class of polymer and the proportion of available ATP allocated to each process were then compared. Protein synthesis consumed the largest proportion of ATP synthesized under all three oxygen regimes, with the proportion of ATP allocated to protein synthesis in anoxia (52%) more than double that in normoxic coleoptiles (19%). Energy allocation to cell wall synthesis was undiminished in hypoxia, consistent with preferential elongation typical of submerged coleoptiles. Lipid synthesis was also conserved strongly in O(2) deficits, suggesting that membrane integrity was maintained under anoxia, thus allowing K(+) to be retained within coleoptile cells. Rates of protein synthesis in coleoptiles from rice cultivars with contrasting tolerance to oxygen deficits (including mutants deficient in fermentative enzymes) confirmed that synthesis and turnover of proteins always accounted for most of the ATP consumed under anoxia. It is concluded that successful establishment of rice seedlings under water is largely due to the capacity of coleoptiles to allocate energy to vital processes, particularly protein synthesis. |
| Databáze: | OpenAIRE |
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