Direct Repression of Cyclin D1 by SIP1 Attenuates Cell Cycle Progression in Cells Undergoing an Epithelial Mesenchymal Transition
| Autor: | Marina Kriajevska, Tatyana Chernova, Jakob Mejlvang, J. Kilian Mellon, Geert Berx, Eugene Tulchinsky, A. Emre Sayan, Cindy Vandewalle |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Transcription
Genetic Cyclin D Cyclin A Cyclin B Down-Regulation Nerve Tissue Proteins Mesoderm Cyclin D1 Cell Line Tumor Humans Epithelial–mesenchymal transition Promoter Regions Genetic Molecular Biology biology Cell Cycle RNA-Binding Proteins Epithelial Cells Articles Cell Biology Cell cycle Science General Cadherins Cell biology Mutation biology.protein A431 cells Cyclin A2 |
| Zdroj: | MOLECULAR BIOLOGY OF THE CELL |
| ISSN: | 1939-4586 1059-1524 |
| DOI: | 10.1091/mbc.e07-05-0406 |
| Popis: | Zinc finger transcription factors of the Snail/Slug and ZEB-1/SIP1 families control epithelial-mesenchymal transitions in development in cancer. Here, we studied SIP1-regulated mesenchymal conversion of epidermoid A431 cells. We found that concomitant with inducing invasive phenotype, SIP1 inhibited expression of cyclin D1 and induced hypophosphorylation of the Rb tumor suppressor protein. Repression of cyclin D1 was caused by direct binding of SIP1 to three sequence elements in the cyclin D1 gene promoter. By expressing exogenous cyclin D1 in A431/SIP1 cells and using RNA interference, we demonstrated that the repression of cyclin D1 gene by SIP1 was necessary and sufficient for Rb hypophosphorylation and accumulation of cells in G1 phase. A431 cells expressing SIP1 along with exogenous cyclin D1 were highly invasive, indicating that SIP1-regulated invasion is independent of attenuation of G1/S progression. However, in another epithelial-mesenchymal transition model, gradual mesenchymal conversion of A431 cells induced by a dominant negative mutant of E-cadherin produced no effect on the cell cycle. We suggest that impaired G1/S phase progression is a general feature of cells that have undergone EMT induced by transcription factors of the Snail/Slug and ZEB-1/SIP1 families. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|