Binding of Cholera Toxin B-Subunit to a Ganglioside GM1-Functionalized PEG-Tethered Lipid Membrane

Autor:	Erik B. Watkins, Andrew J. C. Dennison, Jaroslaw Majewski
Rok vydání:	2022
Předmět:	Cholera Toxin Polymers Lipid Bilayers Electrochemistry General Materials Science G(M1) Ganglioside Surfaces and Interfaces Condensed Matter Physics Spectroscopy Polyethylene Glycols
Zdroj:	Langmuir. 38:6959-6966
ISSN:	1520-5827 0743-7463
Popis:	We report neutron reflectometry (NR) studies of polyethylene glycol (PEG)-tethered model lipid membranes at the solid-liquid interface and of cholera toxin's B-subunit (CTxB) binding to tethered membranes containing ganglioside GM1 receptors. First, tethered polymer brushes were formed by grafting silane-functionalized PEG lipopolymers to quartz from solution. Subsequent deposition of lipids by Langmuir-Blodgett/Langmuir-Schaefer (LB/LS) resulted in a tethered bilayer structure separated from the solid support by a hydrated PEG layer. NR revealed that the tethers formed a highly hydrated polymer brush, uniformly separating the bilayer from the underlying solid substrate. Further, the lipid bilayer did not significantly perturb the brush's conformation relative to a free brush. Biological functionality of the tethered bilayers was verified by interacting CTxB, with ganglioside GM1 receptors incorporated into the bilayer. The surface coverage of CTxB bound to the lipid membrane, θ
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::999adb658bdc9e538468e834157475a7 https://doi.org/10.1021/acs.langmuir.2c00499 Zobrazit plný text záznamu