Expression of a Recombinant Nucleocapsid Protein of Rift Valley Fever Virus in Vero Cells as an Immunofluorescence Antigen and Its Use for Serosurveillance in Traditional Cattle Herds in Zambia
| Autor: | Yona Sinkala, Shuetsu Fukushi, Masahiro Kajihara, Aaron S. Mweene, Shigeru Morikawa, George Dautu, Akina Mori-Kajihara, Kumiko Yoshimatsu, Ngonda Saasa, Ayato Takada, Jiro Arikawa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Rift Valley Fever animal diseases 030231 tropical medicine Fluorescent Antibody Technique Gene Expression Zambia chemical and pharmacologic phenomena Immunofluorescence Antibodies Viral Microbiology Virus 03 medical and health sciences 0302 clinical medicine Antigen Seroepidemiologic Studies Virology parasitic diseases Chlorocebus aethiops medicine Animals Vector (molecular biology) Antigens Viral medicine.diagnostic_test biology Antibody titer virus diseases Nucleocapsid Proteins bacterial infections and mycoses Rift Valley fever virus Recombinant Proteins 030104 developmental biology Infectious Diseases Immunization Epidemiological Monitoring Vero cell biology.protein Cattle Antibody |
| Zdroj: | Vector borne and zoonotic diseases (Larchmont, N.Y.). 18(5) |
| ISSN: | 1557-7759 |
| Popis: | The open reading frame of the nucleocapsid protein (NP) of Rift Valley fever virus (RVFV) strain MP12 was cloned and expressed in Vero E6 cells. The recombinant NP (rNP)-expressing cells were used as antigens for an indirect immunofluorescent antibody assay (IFA). The rNP-based IFA and RVFV-infected Vero E6 cell (authentic antigen)-based IFA showed similar IFA profiles with immune rabbit serum, which was prepared by immunization with rNP expressed using a baculovirus vector. A total of 942 traditional cattle sera obtained in five districts in Central, Southern, and Western provinces of Zambia were screened for anti-RVFV antibodies by the authentic antigen-based and rNP-based IFAs. Significant agreement was obtained between the two IFAs. The findings show that the rNP-based IFA is a safe and useful diagnostic tool as an alternative to the authentic antigen-based IFA. The antibody titers given by the rNP-based IFA were higher than those by the authentic antigen-based IFA. Therefore, the rNP-based IFA might be useful for serosurveillance of RVFV infection among cattle. Antibody prevalence rates in the five districts were 1.3% to 13.5% in the authentic antigen-based IFA and 6.0% to 21.4% in the rNP-based IFA. The results indicated that despite no reports of active cases of RVF in these provinces of Zambia, the virus is circulating among cattle herds. |
| Databáze: | OpenAIRE |
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