Genetic Diversity in Microcystis populations of a French storage reservoir assessed by sequencing of the 16S-23S rRNA intergenic spacer
| Autor: | H. Giraudet, Jean-François Humbert, B. Le Berre, Marie-José Salençon, D. Duris-Latour |
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| Přispěvatelé: | Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL), Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Laboratoire de Biologie Animale Appliquée (LB2A), Université Jean Monnet [Saint-Étienne] (UJM), Electricité de France, Partenaires INRAE |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Geologic Sediments
Microcystis MICROCYSTIS AERUGINOSA Soil Science Population genetics Biology Nucleotide diversity 03 medical and health sciences Water column RNA Ribosomal 16S DNA Ribosomal Spacer 14. Life underwater Ribosomal DNA Ecology Evolution Behavior and Systematics ComputingMilieux_MISCELLANEOUS 030304 developmental biology [SDV.EE]Life Sciences [q-bio]/Ecology environment 0303 health sciences Genetic diversity Ecology 030306 microbiology Genetic Variation Pelagic zone Biodiversity biology.organism_classification RNA Bacterial RNA Ribosomal 23S 16S-23S rRNA Benthic zone France Water Microbiology |
| Zdroj: | Microbial Ecology Microbial Ecology, Springer Verlag, 2005, 49 (2), pp.308-314. ⟨10.1007/s00248-004-0004-z⟩ |
| ISSN: | 0095-3628 1432-184X |
| DOI: | 10.1007/s00248-004-0004-z⟩ |
| Popis: | We compared the genetic diversity of the 16S-23S spacer of the rRNA gene (ITS1) in benthic and pelagic colonies of the Microcystis genus isolated from two different sampling stations with different depths and at two different sampling times (winter and summer) in the French storage reservoir of Grangent. In all, 66 ITS1 sequences were found in the different clone libraries. The nucleotide diversity of all the sampled isolates were in the same range (average number = 0.022) regardless of their origin, showing that several clones are involved in the summer bloom event and contribute to the high biomass production. Phylogenetic study and analysis of molecular variance (AMOVA) revealed no obvious genetic differentiation between the benthic and pelagic isolates. This finding confirms that the Microcystis genus in this lake is characterized by having both a benthic phase in winter and spring allowing this organism to survive in unfavorable environmental conditions, and a pelagic phase in summer and autumn when environmental conditions allow them to grow in the water column. Finally, comparing these sequences with those available in the GenBank database showed that some highly conserved genotypes are found throughout the world. |
| Databáze: | OpenAIRE |
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