Efficient establishment of primary fibroblast cultures from the hawksbill sea turtle (Eretmochelys imbricata)

Autor: Hiroshi Nitto, Katsuhiko Nishimori, Manabu Onuma, Hideko Sone, Takafumi Uchida, Norihisa Oseko, Tomomi Saito, Kei Yuasa, Masanobu Kurita, Tomokazu Fukuda, Jun Kurita, Emiko Isogai, Makoto Soichi, Miho Inoue-Murayama, Kenichiro Donai
Rok vydání: 2012
Předmět:
Zdroj: In Vitro Cellular & Developmental Biology - Animal. 48:660-665
ISSN: 1543-706X
1071-2690
Popis: The hawksbill sea turtle (Eretmochelys imbricata) is a critically endangered species at a risk of extinction. Preservation of the genomic and cellular information of endangered animals is important for future genetic and biological studies. Here, we report the efficient establishment of primary fibroblast cultures from skin tissue of the hawksbill sea turtle. We succeeded in establishing 19 primary cultures from 20 hawksbill sea turtle individuals (a success rate of 95%). These cells exhibited a fibroblast-like morphology and grew optimally at a temperature of 26°C, but experienced a loss of viability when cultured at 37°C. Chromosomal analysis using the primary cells derived here revealed that hawksbill sea turtles have a 2n = 56 karyotype. Furthermore, we showed that our primary cell cultures are free of several fish-related viruses, and this finding is important for preservation purposes. To our knowledge, this report is the first to describe primary cell cultures established from normal tissues of the hawksbill sea turtle. The results will contribute to the preservation of biodiversity, especially for the sea turtles that are critically endangered owing to human activities.
Databáze: OpenAIRE
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