Reduction of polyethylenimine-coated iron oxide nanoparticles induced autophagy and cytotoxicity by lactosylation
| Autor: | Rongrong Jin, Jun Wu, Taipeng Shen, Wencheng Zhu, Changqiang Wu, Jiuju Du, Li Yang, Hua Ai, Bingbing Lin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
autophagy lactosylation RAW 264.7 Nanoparticle macromolecular substances 02 engineering and technology Biomaterials 03 medical and health sciences chemistry.chemical_compound Amphiphile Viability assay Cytotoxicity Research Articles Polyethylenimine Autophagy technology industry and agriculture 021001 nanoscience & nanotechnology 030104 developmental biology chemistry Biochemistry SPIO Drug delivery Biophysics polyethyleneimine 0210 nano-technology Iron oxide nanoparticles MRI |
| Zdroj: | Regenerative Biomaterials |
| ISSN: | 2056-3426 2056-3418 |
| Popis: | Superparamagnetic iron oxide (SPIO) nanoparticles are excellent magnetic resonance contrast agents and surface engineering can expand their applications. When covered with amphiphilic alkyl-polyethyleneimine (PEI), the modified SPIO nanoparticles can be used as MRI visible gene/drug delivery carriers and cell tracking probes. However, the positively charged amines of PEI can also cause cytotoxicity and restricts their further applications. In this study, we used lactose to modify amphiphilic low molecular weight polyethylenimine (C12-PEI2K) at different lactosylation degree. It was found that the N-alkyl-PEI-lactobionic acid wrapped SPIO nanocomposites show better cell viability without compromising their labelling efficacy as well as MR imaging capability in RAW 264.7 cells, comparing to the unsubstituted ones. Besides, we found the PEI induced cell autophagy can be reduced via lactose modification, indicating the increased cell viability might rely on down-regulating autophagy. Thus, our findings provide a new approach to overcome the toxicity of PEI wrapped SPIO nanocomposites by lactose modification. |
| Databáze: | OpenAIRE |
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