Oxidant and SDS-stable alkaline protease from a halo-tolerant Bacillus clausii I-52: enhanced production and simple purification

Autor: H.-S. Joo, C.-S. Chang
Rok vydání: 2005
Předmět:
Zdroj: Journal of Applied Microbiology. 98:491-497
ISSN: 1365-2672
1364-5072
DOI: 10.1111/j.1365-2672.2004.02464.x
Popis: H.-S. JOO AND C.-S. CHANG. 2004. Aims: An investigation was carried out on the enhancement of protease production and simple purification of an oxidant and SDS-stable alkaline protease produced by Bacillus clausii I-52 of industrial significance. Methods and Results: The supplementation with 0AE4% (w/v) NaCl and 0AE05% (w/v) FeSO4AE7H2O in a culture medium caused an increase in the protease production. The enzyme was purified to homogeneity with overall recovery of 79% and 10-fold purification from culture supernatant using Diaion HPA75, phenyl-Sepharose and DEAE-Sepharose column chromatographies. The protease was a halo-tolerant enzyme with apparent molecular mass of 28 kDa, and the Km and kcat values for N-Succinyl-Ala-Ala-Pro-Phe-pNA at 45� C and pH 11AE0 were determined to be 83AE9 lmol l )1 and 238AE 6s )1 respectively. Conclusions: Bacillus clausii I-52 was identified as a halo-tolerant bacterium, and the extracellular alkaline protease produced by B. clausii I-52 also showed extreme halo-tolerance. The enzyme stability towards SDS and H2O2 could be increased by adding NaCl or propylene glycol to the enzyme solution. Significance and Impact of the Study: The alkaline protease secreted by B. clausii I-52 is significant from an industrial perspective because of its stability against surfactants and oxidants as well as its tolerance towards high salinity. These enzymatic properties suggest its suitable application for industrial purposes.
Databáze: OpenAIRE
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