Resveratrol exerts anti-obesity effects in high-fat diet obese mice and displays differential dosage effects on cytotoxicity, differentiation, and lipolysis in 3T3-L1 cells
Autor: | Keng-Yang Lin, Li-Man Hung, Chih-Chun Chang, Yuan-Ji Day, Kang-Yu Peng |
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Rok vydání: | 2016 |
Předmět: |
Male
0301 basic medicine medicine.medical_specialty Lipolysis Endocrinology Diabetes and Metabolism Mice Obese Adipose tissue Resveratrol Diet High-Fat Mice 03 medical and health sciences chemistry.chemical_compound Endocrinology 3T3-L1 Cells Adipocyte Internal medicine Stilbenes medicine Animals Obesity Cytotoxicity Adipogenesis Cell Death Dose-Response Relationship Drug food and beverages Cell Differentiation Mice Inbred C57BL 030104 developmental biology chemistry Perilipin Tumor necrosis factor alpha Anti-Obesity Agents hormones hormone substitutes and hormone antagonists |
Zdroj: | Endocrine Journal. 63:169-178 |
ISSN: | 1348-4540 0918-8959 |
Popis: | Studies on resveratrol in a wide range of concentrations on obese mice and adipose cells are necessary to comprehend its range of diverse and contradictory effects. In this study, we examined the anti-obesity effects of resveratrol on high-fat diet (HFD)-induced obese mice at dosages ranging from 1 to 30 mg/kg treatment for 10 wk. We also evaluated the effects of resveratrol on cytotoxicity, proliferation, adipogenic differentiation, and lipolysis of 3T3-L1 cells at concentrations ranging from 0.03 to 100 μM. In HFD obese mice, resveratrol treatment for 10 wk without decreased calories intake significantly attenuated HFD-induced weight gain in a dose-dependent manner. Resveratrol treatment also protected against HFD-induced lipid deposition in adipose tissues and liver. In cultured 3T3-L1 preadipocytes, high dosage (10 to 100 μM) resveratrol treatment produced cytotoxicity in both preadipocytes and mature adipocytes. In contrast, low concentration resveratrol treatment (1 to 10 μM) significantly inhibited the capacity of 3T3-L1 cells differentiated into mature adipocytes. Low dose resveratrol treatment also downregulated peroxisome proliferator-activated receptor gamma (PPARγ) and perilipin protein expressions in differentiated adipocytes. Additionally, tumor necrosis factor alpha (TNFα)-induced lipolysis was inhibited by low concentration resveratrol treatment in mature adipocytes. At concentrations of 10-100 μM, resveratrol exerted cytotoxicity. In contrast, at concentrations of 1-10 μM resveratrol inhibited adipogenic differentiation in preadipocytes and suppressed lipolysis in mature adipocytes. Our results suggest that resveratrol possessed anti-obesity effects by induction of cytotoxicity at high dosage and that it influences preadipocyte differentiation and mature adipocyte lipolysis at low concentration. |
Databáze: | OpenAIRE |
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