Identification of the proteins specifically binding to the rat LINE1 promoter
| Autor: | Olga I. Podgornaya, Dmitry V. Lukyanov, Anton V. Fedorov |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Untranslated region Sp1 Transcription Factor Molecular Sequence Data Response element Biophysics CAAT box Plasma protein binding Biochemistry Sp3 transcription factor Transcription (biology) Testis Animals Promoter Regions Genetic Southwestern blot Molecular Biology Sp1 transcription factor Base Sequence Chemistry Sequence Analysis DNA Cell Biology Molecular biology Rats Long Interspersed Nucleotide Elements Sp3 Transcription Factor Molecular Probes Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 340:553-559 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2005.12.040 |
| Popis: | The initial step of LINE1 retrotransposons dissemination requires transcription from species-specific promoter located within 5'-untranslated region of LINE1. Although the 5'-untranslated region of the rat LINE1 element shows promoter activity, no promoter-binding proteins have been discovered so far. Using an EMSA and Southwestern blotting methods, we identified Sp1 and Sp3 proteins, which specifically bind to the rat LINE1 promoter in vitro. The Sp1/Sp3-binding motif within rat LINE1 promoter is located downstream of the major predicted transcription initiation site. |
| Databáze: | OpenAIRE |
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