Distinct Signaling by Ventral Tegmental Area Glutamate, GABA, and Combinatorial Glutamate-GABA Neurons in Motivated Behavior
| Autor: | Karl Deisseroth, David J. Barker, David H. Root, Bing Liu, David J. Estrin, Yoon Seok Kim, Francois Vautier, Jorge Miranda-Barrientos, Shiliang Zhang, Marisela Morales, Lief E. Fenno, Charu Ramakrishnan, Hui Ling Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Cell type Glutamic Acid Biology Article General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine mental disorders Recombinase medicine Animals Humans GABAergic Neurons Motivation musculoskeletal neural and ocular physiology Ventral Tegmental Area Glutamate receptor Ventral tegmental area 030104 developmental biology medicine.anatomical_structure nervous system Neuroscience psychological phenomena and processes 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Cell reports |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY Ventral tegmental area (VTA) neurons play roles in reward and aversion. We recently discovered that the VTA has neurons that co-transmit glutamate and GABA (glutamate-GABA co-transmitting neurons), transmit glutamate without GABA (glutamate-transmitting neurons), or transmit GABA without glutamate (GABA-transmitting neurons). However, the functions of these VTA cell types in motivated behavior are unclear. To identify the functions of these VTA cell types, we combine recombinase mouse lines with INTRSECT2.0 vectors to selectively target these neurons. We find that VTA cell types have unique signaling patterns for reward, aversion, and learned cues. Whereas VTA glutamate-transmitting neurons signal cues predicting reward, VTA GABA-transmitting neurons signal cues predicting the absence of reward, and glutamate-GABA co-transmitting neurons signal rewarding and aversive outcomes without signaling learned cues related to those outcomes. Thus, we demonstrate that genetically defined subclasses of VTA glutamate and GABA neurons signal different aspects of motivated behavior. Graphical Abstract In Brief Root et al. examine ventral tegmental area (VTA) neurons that release glutamate without GABA, GABA without glutamate, or both glutamate and GABA. Cell types have differential projection densities and unique neuronal activity profiles related to cues predicting rewarding, nonreward, or aversive outcomes and in the receipt of predicted rewards. |
| Databáze: | OpenAIRE |
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