Arachidonic acid in mononuclear cells and its clinical significance in HCV cirrhotic patients

Autor: Nobuyuki Sakai, Toshiya Osawa, Mitsuhiko Kawaguchi, Tatsuya Itoshima, Kayoko Tomioka, Misako Okita, Takayo Sasagawa, Yasuko Ota
Rok vydání: 2003
Předmět:
Liver Cirrhosis
Male
medicine.medical_specialty
Carcinoma
Hepatocellular
Chromatography
Gas
Cirrhosis
Endocrinology
Diabetes and Metabolism
Hepatitis C virus
Lymphocyte
alpha-Tocopherol
Enzyme-Linked Immunosorbent Assay
Biology
medicine.disease_cause
Thiobarbituric Acid Reactive Substances
Gastroenterology
Peripheral blood mononuclear cell
chemistry.chemical_compound
Internal medicine
medicine
Humans
Lymphocyte Count
Chromatography
High Pressure Liquid
Phospholipids
Aged
chemistry.chemical_classification
Arachidonic Acid
Nutrition and Dietetics
Platelet Count
Tumor Necrosis Factor-alpha
Fatty Acids
Liver Neoplasms
Fatty acid
Hepatitis C
Middle Aged
medicine.disease
medicine.anatomical_structure
chemistry
Case-Control Studies
Hepatocellular carcinoma
Immunology
Disease Progression
Leukocytes
Mononuclear
Female
Arachidonic acid
Zdroj: Nutrition. 19:727-732
ISSN: 0899-9007
DOI: 10.1016/s0899-9007(03)00095-9
Popis: An abnormal fatty acid pattern in patients with advanced liver cirrhosis (LC) has been reported in plasma phospholipids and some other tissues. To elucidate the significance of arachidonic acid deficiency on the clinical pathophysiology of LC and hepatocellular carcinoma (HCC), we analyzed the fatty acid compositions of mononuclear cell phospholipids, plasma alpha-tocopherol, and thiobarbituric acid-reactive substances and serum tumor necrosis factor-alpha (TNF-alpha) in cirrhotic patients infected with the hepatitis C virus with and without HCC.Twelve cirrhotic patients without HCC (LC patients) and 11 with HCC (HCC patients) were enrolled. Fatty acids were analyzed with gas chromatography. alpha-Tocopherol and TNF-alpha were analyzed by high-performance liquid chromatography and enzyme-linked immunosorbent assay, respectively. Statistical analysis was performed by using the unpaired t test with Welch's correction and Spearman's rank-correlation analysis.Significantly low levels of linoleic, dihomo-gamma-linolenic, arachidonic, and eicosapentaenoic acids from mononuclear cell phospholipids were observed in LC and HCC patients compared with control subjects. Plasma alpha-tocopherol was lower and thiobarbituric acid-reactive substances were higher in HCC patients than in controls. Arachidonic acid molar percentage in mononuclear cell phospholipids correlated significantly with lymphocyte count (r = 0.460, P0.05) in the cirrhotic patients and with lymphocyte (r = 0.680, P0.01) and platelet (r = 0.763, P0.01) counts in all subjects.These results suggested that arachidonic acid in mononuclear cells may have an important role in the pathophysiology of hepatitis C virus associated with cirrhosis and that nutritional management preventing arachidonic acid deficiency may have some beneficial effects on the progression of LC.
Databáze: OpenAIRE
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