Validation and Comparison of Two Calibration Methods for the Measurement of Stable Iodine in the Urinary Matrix by ICP-MS: Standard Addition vs. External Calibration
| Autor: | Thibaud Sontag, Alexandre Legrand, Grégory Finance, François Rebière, Xavier Millot, Géraldine Landon, Michelle Agarande, Valérie Renaud-Salis, Maïlie Saint-Hilaire, C. Bouvier-Capely |
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| Přispěvatelé: | Institut de Radioprotection et de Sûreté Nucléaire (IRSN), Commissariat à l'énergie atomique et aux énergies alternatives (CEA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Wilcoxon signed-rank test Calibration (statistics) External Calibration [SDV]Life Sciences [q-bio] Analytical chemistry chemistry.chemical_element Context (language use) Standard Addition Urine Iodine Matrix (chemical analysis) 03 medical and health sciences 0302 clinical medicine Statistics Validation Inductively coupled plasma mass spectrometry Mathematics Detection limit Stable Iodine 6. Clean water 3. Good health Psychiatry and Mental health 030104 developmental biology chemistry 030220 oncology & carcinogenesis Standard addition |
| Zdroj: | American Journal of Analytical Chemistry American Journal of Analytical Chemistry, Scientific Research, 2017, 08 (04), pp.245-263. ⟨10.4236/ajac.2017.84019⟩ American Journal of Analytical Chemistry, 2017, 08 (04), pp.245-263. ⟨10.4236/ajac.2017.84019⟩ |
| ISSN: | 2156-8251 2156-8278 |
| DOI: | 10.4236/ajac.2017.84019⟩ |
| Popis: | International audience; Background : In the context of a nuclear reactor accident, thyroid is the main target organ of radioactive iodines. To avoid as much as possible thyroid disorders or even cancer development, it is recommended to administer a single dose of potassium iodide to people at risk of exposure. Nevertheless, the Fu-kushima Dai-ichi disaster has pointed out many questions about the conditions of stable iodine prophylaxis implementation highlighting the need for reflection further revision of the actual "iodine doctrine". Therefore, providing useful data is required notably through the implementation of animal experiments to strengthen current knowledge and to edit new recommendations. Methods : Urinary iodine constitutes a very good indicator to investigate the function of thyroid, its interpretation demands reliable analyses. Prior to perform animal experiments, two calibration methods were designed by our lab and compared together (standard addition and external calibration) to assess the urinary concentration of stable iodine in urine by ICP-MS. They were validated based on several key parameters especially linearity, accuracy and limits of detection (LOD) and quantification (LOQ). Results : The results were nicely satisfying. Indeed, both calibration methods have indicated very good coefficients of correlations, accuracies with low expanded relative uncertainties were obtained. The estimated LOD in the sample for standard addition method and external calibration were fully acceptable, 0.39 µg·L −1 and 0.35 µg·L −1 , respectively. All performance criteria have been thus fulfilled successfully. The established methods were proven to be accurate, robust and sensitive. Once validated, both calibration methods were applied to rat urine samples and the results of z-score and Wilcoxon W test concluded that there were no statistically significant differences between both methods. |
| Databáze: | OpenAIRE |
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