High-Resolution Metabolomics Assessment of Military Personnel
| Autor: | Ken H. Liu, Timothy M. Mallon, Vilinh Tran, Dean P. Jones, Douglas I. Walker, Patricia Rohrbeck, Karan Uppal |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Databases Factual Computer science Electrospray ionization High resolution Computational biology Mass spectrometry 01 natural sciences Mass Spectrometry Article 03 medical and health sciences Metabolomics Occupational Exposure Humans KEGG 010401 analytical chemistry Public Health Environmental and Occupational Health Environmental Exposure Environmental exposure 0104 chemical sciences Military Personnel 030104 developmental biology Ultrahigh resolution Blood Banks Occupational exposure |
| Zdroj: | Journal of Occupational & Environmental Medicine. 58:S53-S61 |
| ISSN: | 1076-2752 |
| DOI: | 10.1097/jom.0000000000000773 |
| Popis: | OBJECTIVE The aim of this study was to maximize detection of serum metabolites with high-resolution metabolomics (HRM). METHODS Department of Defense Serum Repository (DoDSR) samples were analyzed using ultrahigh resolution mass spectrometry with three complementary chromatographic phases and four ionization modes. Chemical coverage was evaluated by number of ions detected and accurate mass matches to a human metabolomics database. RESULTS Individual HRM platforms provided accurate mass matches for up to 58% of the KEGG metabolite database. Combining two analytical methods increased matches to 72% and included metabolites in most major human metabolic pathways and chemical classes. Detection and feature quality varied by analytical configuration. CONCLUSIONS Dual chromatography HRM with positive and negative electrospray ionization provides an effective generalized method for metabolic assessment of military personnel. |
| Databáze: | OpenAIRE |
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