Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameters

Autor: Vesna Prosinecki, Ana Sofia Carvalho, Atul Malhotra, Deborah Penque, Rita da Silva, Cristina Valentim-Coelho, Cristina Bárbara, Rune Matthiesen, Bruno M. Alexandre, Amélia Feliciano, Paula Pinto, Fátima Vaz, Vukosava Milic Torres
Přispěvatelé: Repositório da Universidade de Lisboa
Rok vydání: 2017
Předmět:
Proteomics
Male
0301 basic medicine
Genomica Funcional
Erythrocytes
Proteome
Medical Biochemistry and Metabolomics
Red blood cells
medicine.disease_cause
Severity of Illness Index
0302 clinical medicine
Positive airway pressure
Medicine
Morning
Sleep Apnea
Obstructive
Continuous Positive Airway Pressure
Middle Aged
Peroxiredeoxin-2
3. Good health
Genómica Funcional e Estrutural
Red Blood Cell
Molecular Medicine
Biomarker (medicine)
Oxidation-Reduction
Adult
Biochemistry & Molecular Biology
medicine.medical_specialty
Sleep Apnea
Evening
Sleep Apneia
Photoperiod
Polysomnography
Clinical Sciences
Obstructive Sleep Apne
Peroxiredoxin 2
Article
03 medical and health sciences
Internal medicine
Humans
Molecular Biology
Obstructive
business.industry
Peroxiredoxins
medicine.disease
Obstructive sleep apnea
respiratory tract diseases
Oxidative Stress
030104 developmental biology
Endocrinology
Metabolic Disorders
Biochemistry and Cell Biology
Protein Multimerization
business
Biomarkers
030217 neurology & neurosurgery
Homeostasis
Oxidative stress
Zdroj: Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP
Biochimica et biophysica acta. Molecular basis of disease, vol 1863, iss 2
Feliciano, A; Vaz, F; Torres, VM; Valentim-Coelho, C; Silva, R; Prosinecki, V; et al.(2017). Evening and morning peroxiredoxin-2 redox/oligomeric state changes in obstructive sleep apnea red blood cells: Correlation with polysomnographic and metabolic parameters. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1863(2), 621-629. doi: 10.1016/j.bbadis.2016.11.019. UC San Diego: Retrieved from: http://www.escholarship.org/uc/item/2ff051tz
ISSN: 0925-4439
Popis: © 2016 Published by Elsevier B.V.
We have examined the effects of Obstructive Sleep Apnea (OSA) on red blood cell (RBC) proteome variation at evening/morning day time to uncover new insights into OSA-induced RBC dysfunction that may lead to OSA manifestations. Dysregulated proteins mainly fall in the group of catalytic enzymes, stress response and redox regulators such as peroxiredoxin 2 (PRDX2). Validation assays confirmed that at morning the monomeric/dimeric forms of PRDX2 were more overoxidized in OSA RBC compared to evening samples. Six month of positive airway pressure (PAP) treatment decreased this overoxidation and generated multimeric overoxidized forms associated with chaperone/transduction signaling activity of PRDX2. Morning levels of overoxidized PRDX2 correlated with polysomnographic (PSG)-arousal index and metabolic parameters whereas the evening level of disulfide-linked dimer (associated with peroxidase activity of PRDX2) correlated with PSG parameters. After treatment, morning overoxidized multimer of PRDX2 negatively correlated with fasting glucose and dopamine levels. Overall, these data point toward severe oxidative stress and altered antioxidant homeostasis in OSA RBC occurring mainly at morning time but with consequences till evening. The beneficial effect of PAP involves modulation of the redox/oligomeric state of PRDX2, whose mechanism and associated chaperone/transduction signaling functions deserves further investigation. RBC PRDX2 is a promising candidate biomarker for OSA severity and treatment monitoring, warranting further investigation and validation.
Project partially supported by Harvard Medical School-Portugal Program (HMSPICJ/0022/2011), ToxOmics - Centre for Toxicogenomics and Human Health (FCT-UID/BIM/00009/2013), FCT/Poly-Annual Funding Program and FEDER/Saúde XXI Program (Portugal) and postdoctoral fellowship (SFRH/BPD/43365/2008) of Fundação para a Ciência e a Tecnologia (FCT), Portugal.
Databáze: OpenAIRE
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