V3 loops of HIV-1 specimens from pregnant women in Malawi uniformly lack a potential N-linked glycosylation site
| Autor: | Paolo G. Miotti, Gina Dallabetta, Gerald Schochetman, Gregg M. Orloff, Karen E. Potts, Marcia L. Kalish, Alfred I. Saah, Chin-Yih Ou, John D. Chiphangwi |
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| Rok vydání: | 1993 |
| Předmět: |
Malawi
Glycosylation Immunology Molecular Sequence Data HIV Infections V3 loop Biology HIV Envelope Protein gp120 DNA sequencing Virus chemistry.chemical_compound N-linked glycosylation Valine Pregnancy Virology Humans Amino Acid Sequence Pregnancy Complications Infectious Gene Peptide Fragments Infectious Diseases chemistry HIV-1 Female Nested polymerase chain reaction |
| Zdroj: | AIDS research and human retroviruses. 9(7) |
| ISSN: | 0889-2229 |
| Popis: | The HIV-1 env gene was amplified from the peripheral blood mononuclear cells of 14 infected pregnant women in Malawi. Nested polymerase chain reaction (PCR) and DNA sequencing were performed. The PCR product was purified and the C2-V3 region sequenced. Using the similarity function of the multiple aligned sequence editor, 13 of the nucleotide sequences were compared. The interperson variation, based on single base substitutions, ranged from 7.3 to 22.2% (mean 13.6%). All of the sequences showed the tetrapeptide motif at the crown of the V3 loop which is commonly seen among HIV-1 subtypes A, C, D, and E. The C2-V3 coding sequences clustered with the subtype C sequence reported from South Africa. In addition, all of these sequences lacked a potential N-linked glycosylation site found in all HIV-1 sequences except subtype C. In these specimens, the predominant replacement was valine. The role of this site in HIV-1 transmission is controversial. |
| Databáze: | OpenAIRE |
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