Immunological identification of the alternative oxidase ofAcanthamoeba castellaniimitochondria
| Autor: | Marijke J. Wagner, Anneke M. Wagner, Wieslawa Jarmuszkiewicz, Lilla Hryniewiecka |
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| Rok vydání: | 1997 |
| Předmět: |
Alternative oxidase
food.ingredient Cyanide-resistant respiration (Acanthamoeba castellanii) Immunoblotting Biophysics Acanthamoeba Biology Mitochondrion Biochemistry Microbiology Maleimides Mitochondrial Proteins Amoeba (genus) chemistry.chemical_compound food Structural Biology parasitic diseases Genetics Animals Molecular Biology Plant Proteins Diamide Oxidase test GMP Antibodies Monoclonal Cell Biology biology.organism_classification Salicylhydroxamic acid Mitochondria Dithiothreitol Cross-Linking Reagents chemistry Cell culture Protozoa Acanthamoeba castellanii Oxidoreductases |
| Zdroj: | FEBS Letters. 411:110-114 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(97)00676-5 |
| Popis: | Mitochondria of the protozoa Acanthamoeba castellanii possess a cyanide-insensitive oxidase cross-reacting with monoclonal antibodies raised against the plant alternative oxidase. Immunoblotting revealed three monomeric forms (38, 35, and 32 kDa) and very low amounts of a single 65 kDa dimeric form. Cross-linking studies suggest that while in plants the alternative oxidase occurs as a dimer, in amoeba it functions as a monomer. Immunologically detectable protein levels change with the age of amoeba cell culture. Increased amounts of the 35 kDa protein are accompanied by an increase in the activity of cyanide-resistant respiration. |
| Databáze: | OpenAIRE |
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