Crystal and Supramolecular Structure of Bacterial Cellulose Hydrolyzed by Cellobiohydrolase from Scytalidium Candidum 3C: A Basis for Development of Biodegradable Wound Dressings
| Autor: | Nikolay Verlov, Marat S. Asadulaev, Anna A. Kulminskaya, K. B. Ustinovich, Yulia Gorshkova, Alexander Ye. Baranchikov, Andrey M. Fedyk, T. V. Khamova, Gennady P. Kopitsa, Lyubov A. Ivanova, Anton S. Shabunin, Elena V. Eneyskaya, E V Zinov'ev, Vladimir Burdakov, Natalia V. Tsvigun |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Scanning electron microscope
02 engineering and technology macromolecular substances 010402 general chemistry wound dressing 01 natural sciences lcsh:Technology Crystal Hydrolysis chemistry.chemical_compound meso- and microstructure Enzymatic hydrolysis Specific surface area cellobiohydrolase General Materials Science lcsh:Microscopy lcsh:QC120-168.85 lcsh:QH201-278.5 Chemistry bacterial cellulose lcsh:T technology industry and agriculture enzymatic hydrolysis Biodegradation 021001 nanoscience & nanotechnology Microstructure 0104 chemical sciences Bacterial cellulose lcsh:TA1-2040 lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering 0210 nano-technology lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 ddc:600 Nuclear chemistry |
| Zdroj: | Materials 13(9), 2087-(2020). doi:10.3390/ma13092087 Materials, Vol 13, Iss 2087, p 2087 (2020) Materials Volume 13 Issue 9 |
| Popis: | The crystal and supramolecular structure of the bacterial cellulose (BC) has been studied at different stages of cellobiohydrolase hydrolysis using various physical and microscopic methods. Enzymatic hydrolysis significantly affected the crystal and supramolecular structure of native BC, in which the 3D polymer network consisted of nanoribbons with a thickness T &asymp 8 nm and a width W &asymp 50 nm, and with a developed specific surface SBET &asymp 260 m2· g&minus 1. Biodegradation for 24 h led to a ten percent decrease in the mean crystal size Dhkl of BC, to two-fold increase in the sizes of nanoribbons, and in the specific surface area SBET up to &asymp 100 m2· 1. Atomic force and scanning electron microscopy images showed BC microstructure &ldquo loosening&ldquo after enzymatic treatment, as well as the formation and accumulation of submicron particles in the cells of the 3D polymer network. Experiments in vitro and in vivo did not reveal cytotoxic effect by the enzyme addition to BC dressings and showed a generally positive influence on the treatment of extensive III-degree burns, significantly accelerating wound healing in rats. Thus, in our opinion, the results obtained can serve as a basis for further development of effective biodegradable dressings for wound healing. |
| Databáze: | OpenAIRE |
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