The structure of the cyanobactin domain of unknown function from PatG in the patellamide gene cluster
| Autor: | Marcel Jaspars, Jesko Koehnke, Rachael Graham, Wael E. Houssen, Andrew F. Bent, Greg Mann, James H. Naismith, Uli Schwarz-Linek |
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| Přispěvatelé: | BBSRC, University of St Andrews. School of Chemistry, University of St Andrews. School of Biology, University of St Andrews. Biomedical Sciences Research Complex, University of St Andrews. EaSTCHEM |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Protein Conformation
Molecular Sequence Data Protein domain Biophysics Computational biology Biology Peptides Cyclic Biochemistry chemistry.chemical_compound Protein structure Biosynthesis Structural Biology cyanobactins Gene cluster Hydrolase Genetics Structural Communications QD Amino Acid Sequence PatG Nuclear Magnetic Resonance Biomolecular chemistry.chemical_classification Sequence Homology Amino Acid Condensed Matter Physics QD Chemistry Cyclic peptide patellamides RiPPs chemistry Cyclization Domain of unknown function Function (biology) |
| Zdroj: | Acta Crystallographica. Section F, Structural Biology Communications |
| ISSN: | 2053-230X |
| Popis: | The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated. |
| Databáze: | OpenAIRE |
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