| Autor: |
Campanella, Jonatas Erick Maimoni, Candido, Thiago de Souza, Barbosa, Luiz Carlos Bertucci, Gomes, Antoniel Augusto Severo, Leite, Carla Andréa, Higashi, Erika Silva, Barbugli, Paula Aboud, Fontes, Marcos Roberto de Matos, Bertolini, Maria Célia |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Frontiers in Microbiology. 13 |
| ISSN: |
1664-302X |
| DOI: |
10.3389/fmicb.2022.1078972 |
| Popis: |
Cyclins are a family of proteins characterized by possessing a cyclin box domain that mediates binding to cyclin dependent kinases (CDKs) partners. In this study, the search for a partner cyclin of the PHO85-1 CDK retrieved PCL-1 an ortholog of yeast Pcls (for Pho85 cyclins) that performs functions common to Pcls belonging to different cyclin families. We show here that PCL-1, as a typical cyclin, is involved in cell cycle control and cell progression. In addition, PCL-1 regulates glycogen metabolism; Δpcl-1 cells accumulate higher glycogen levels than wild-type cells and the glycogen synthase (GSN) enzyme is less phosphorylated and, therefore, more active in the mutant cells. Together with PHO85-1, PCL-1 phosphorylates in vitro GSN at the Ser636 amino acid residue. Modeling studies identified PHO85-1 and PCL-1 as a CDK/cyclin complex, with a conserved intermolecular region stabilized by hydrophobic and polar interactions. PCL-1 is also involved in calcium and NaCl stress response. Δpcl-1 cells are sensitive to high NaCl concentration; on the contrary, they grow better and overexpress calcium responsive genes under high calcium chloride concentration compared to the wild-type strain. The expression of the calcium-responsive CRZ-1 transcription factor is modulated by PCL-1, and this transcription factor seems to be less phosphorylated in Δpcl-1 cells since exhibits nuclear location in these cells in the absence of calcium. Our results show that PCL-1 locates at different cell regions suggesting that it may determine its activity by controlling its intracellular location and reveal an interesting functional divergence between yeast and filamentous fungus cyclins. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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