Quantification of telomerase activity in normal oral mucosal tissue and oral squamous cell carcinoma
| Autor: | Venkatesh G Naikmasur, Arpita Rai, Atul P. Sattur |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
squamous cell carcinoma Telomerase medicine.medical_specialty Pathology Malignancy telomerase Gastroenterology law.invention Lesion 03 medical and health sciences 0302 clinical medicine law Internal medicine Medicine Polymerase chain reaction telomere Cancer prevention business.industry Cancer Biomarker medicine.disease Telomere stomatognathic diseases 030104 developmental biology Oncology 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Biomarker (medicine) Original Article medicine.symptom business |
| Zdroj: | Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology |
| ISSN: | 0975-2129 0971-5851 |
| Popis: | Background and Objective: The role of telomeres and telomerase in oral cancer is an area of much recent interest. The understanding of the role of telomere biology, the end replication problem leading to genomic instability and the reactivation of telomerase, is absolutely critical to our understanding of oral cancer, and more so, to our ability of early diagnosis and developing novel therapies and cancer prevention approaches. The aim of the present study was to quantify telomerase activity (TA) in oral squamous cell carcinoma (OSCC) and normal oral mucosa and assess the role of telomerase as diagnostic and prognostic marker of oral malignancy. Materials and Methods: We quantified TA in 45 patients with OSCC and 20 normal oral mucosal specimens using polymerase chain reaction-based telomeric repeat amplification protocol assay and compared it with the clinical status and grade of malignancy. Results: TA was detected in 89% of malignant and 5% of normal oral mucosal tissue. The TA levels ranged from 0.28 to 6.91 (mean 2.05, standard deviation [SD] 1.33) in OSCC and 0.21 to 1.09 (mean 0.54, SD 0.27) in normal oral mucosa. There was no relationship between TA levels and clinical stages, site of the lesion, history of adverse habits, or sex of the patient. However, under the WHO classification, there were significant differences P < 0.00) between Grades I, II, and III. Furthermore, increasing age of the patient significantly correlated with TA. Interpretation and Conclusion: The results of the present study indicate that activation of TA is frequent in OSCC. Statistically significant difference in quantified telomerase levels of OSCC and normal oral mucosa P < 0.00) demonstrates the significant clinical usefulness of telomerase activation as a valuable marker for diagnosis while significant correlation of TA with grades of malignancy indicates its effectiveness as marker for prognosis of OSCC. |
| Databáze: | OpenAIRE |
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