RotundRacGAP functions with ras during spermatognesis and retinal differentiation in Drosophila melanogaster
| Autor: | Karine Raymond, Michel Cazemajor, Ruth Griffin-Shea, Isabelle Pignot-Paintrand, Evelyne Bergeret, Annabel Guichard, Marie-Odile Fauvarque |
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| Přispěvatelé: | Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Carret, Michèle, Transduction du signal : signalisation calcium, phosphorylation et inflammation, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), inconnu, Inconnu, Griffin, Ruth |
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Male
GTPase-activating protein MESH: Drosophila Proteins Genes Insect MESH: GTPase-Activating Proteins MESH: Genes Insect Biology Retina MESH: Drosophila melanogaster 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine [SDV.BDD] Life Sciences [q-bio]/Development Biology MESH: Gene Expression Regulation Developmental medicine Animals Drosophila Proteins MESH: Animals Spermatogenesis Cell Growth and Development Molecular Biology [SDV.BDD]Life Sciences [q-bio]/Development Biology ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences Spermatid MESH: Retina GTPase-Activating Proteins Gene Expression Regulation Developmental Spermatid differentiation Retinal Cell Biology biology.organism_classification Null allele Phenotype MESH: Male Cell biology Drosophila melanogaster medicine.anatomical_structure chemistry ras Proteins MESH: Spermatogenesis MESH: ras Proteins 030217 neurology & neurosurgery |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2001, pp.6280-6291 Molecular and Cellular Biology Molecular and Cellular Biology, American Society for Microbiology, 2001, 21 (18), pp.6280-91 Molecular and Cellular Biology, 2001, 21 (18), pp.6280-91 |
| ISSN: | 1939-4586 0270-7306 1098-5549 |
| Popis: | International audience; Our analysis of rotund (rn) null mutations in Drosophila melanogaster revealed that deletion of the rn locus affects both spermatid and retinal differentiation. In the male reproductive system, the absence of RnRacGAP induced small testes, empty seminal vesicles, short testicular cysts, reduced amounts of interspermatid membrane, the absence of individualization complexes, and incomplete mitochondrial condensation. Flagellar growth continued within the short rn null cysts to produce large bulbous terminations of intertwined mature flagella. Organization of the retina was also severely perturbed as evidenced by grossly misshapen ommatidia containing reduced numbers of photoreceptor and pigment cells. These morphological phenotypes were rescued by genomic rnRacGAP transgenes, demonstrating that RnRacGAP function is critical to spermatid and retinal differentiation. The testicular phenotypes were suppressed by heterozygous hypomorphic mutations in the Dras1 and drk genes, indicating cross talk between RacGAP-regulated signaling and that of the Ras pathway. The observed genetic interactions are consistent with a model in which Rac signaling is activated by Ras and negatively regulated by RnRacGAP during spermatid differentiation. RnRacGAP and Ras cross talk also operated during retinal differentiation; however, while the heterozygous hypomorphic drk mutation continued to act as a suppressor of the rn null mutation, the heterozygous hypomorphic Dras1 mutation induced novel retinal phenotypes. |
| Databáze: | OpenAIRE |
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