A mouse model of humanized liver shows a human-like lipid profile, but does not form atherosclerotic plaque after western type diet
| Autor: | Andre Boonstra, Maikel P. Peppelenbosch, Jeanine E. Roeters van Lennep, Gulce Sari, Kim van der Heiden, Leonie C. van Vark-van der Zee, Kristiaan Wouters, Monique T. Mulder, Thomas Vanwolleghem, Eric Jan Meester |
|---|---|
| Přispěvatelé: | Gastroenterology & Hepatology, Cardiology, Radiology & Nuclear Medicine, Internal Medicine, Interne Geneeskunde, RS: Carim - V01 Vascular complications of diabetes and metabolic syndrome |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Biophysics Human liver chimeric mouse model VIRUS-INFECTION Biology Biochemistry THERAPY Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lipoprotein synthesis Time frame In vivo Internal medicine medicine Animals Humans C VIRUS Lipoprotein Molecular Biology medicine.diagnostic_test Cholesterol Physics High fat diet CHIMERIC MICE Cell Biology Metabolism Lipid Metabolism Atherosclerosis Cardiovascular disease Lipids Plaque Atherosclerotic Pathophysiology Disease Models Animal Chemistry 030104 developmental biology Endocrinology HUMAN HEPATOCYTES Liver chemistry Diet Western 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) Human medicine Lipid profile |
| Zdroj: | Biochemical and Biophysical Research Communications, 524, 510-515. Academic Press Biochemical and Biophysical Research Communications, 524(2), 510-515. Elsevier Biochemical and biophysical research communications |
| ISSN: | 0006-291X |
| Popis: | Mouse models are a crucial and often used tool to provide insight into the underlying mechanisms of human atherosclerosis. However, mice profoundly differ from humans in lipoprotein synthesis and metabolism, key factors in atherosclerotic plaque formation. Mouse models often require genetic and dietary modifications to mimic human pathophysiology, shifting from a high-density lipoprotein to an low-density lipoprotein dominant lipoprotein profile. We examined the suitability of mice with a humanized liver as a model for lipoprotein studies and studies on plaque formation, given the central role of hepatocytes in lipoprotein synthesis and metabolism. Our results show a progressive humanization of the mouse liver and a humanized lipoprotein profile. However, no atherosclerotic plaque formation was observed in the studied time frame, despite presence of functional macrophages and application of a high cholesterol western-type diet. The humanized-liver mouse model therefore might require further modifications to induce atherosclerosis, yet seems a valuable model for in vivo studies on lipoprotein metabolism. (C) 2020 The Authors. Published by Elsevier Inc. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect