Three-Dimensional Printed Scaffolds with Controlled Micro-/Nanoporous Surface Topography Direct Chondrogenic and Osteogenic Differentiation of Mesenchymal Stem Cells
| Autor: | Kevin M. Shakesheff, Mick Cooper, Jing Yang, Aruna Prasopthum |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Cytoskeleton organization Surface Properties Polyesters 3D printing Nanotechnology 02 engineering and technology 03 medical and health sciences Nanopores Osteogenesis Cell Adhesion Humans General Materials Science Polymer scaffold Cells Cultured 030304 developmental biology Cell Proliferation 0303 health sciences Tissue Scaffolds Nanoporous business.industry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Adhesion 021001 nanoscience & nanotechnology Chondrogenesis Porous scaffold Printing Three-Dimensional Thermogravimetry Solvents 0210 nano-technology business |
| Zdroj: | ACS applied materialsinterfaces. 11(21) |
| ISSN: | 1944-8252 |
| Popis: | The effect of topography in three-dimensional (3D) printed polymer scaffolds on stem cell differentiation is a significantly underexplored area. Compared to two-dimensional (2D) biomaterials on which various well-defined topographies have been incorporated and shown to direct a range of cell behaviors including adhesion, cytoskeleton organization, and differentiation, incorporating topographical features to 3D polymer scaffolds is challenging due to the difficulty of accessing the inside of a porous scaffold. Only the roughened strut surface has been introduced to 3D printed porous scaffolds. Here, a rapid, single-step 3D printing method to fabricate polymeric scaffolds consisting of microstruts (ca. 60 μm) with micro-/nanosurface pores (0.2-2.4 μm) has been developed based on direct ink writing of an agitated viscous polymer solution. The density, size, and alignment of these pores can be controlled by changing the degree of agitation or the speed of printing. Three-dimensional printed scaffolds with micro-/nanoporous struts enhanced chondrogenic and osteogenic differentiation of mesenchymal stem cells (MSCs) without soluble differentiation factors. The topography also selectively affected adhesion, morphology, and differentiation of MSC to chondrogenic and osteogenic lineages depending on the composition of the differentiation medium. This fabrication method can potentially be used for a wide range of polymers where desirable architecture and topography are required. |
| Databáze: | OpenAIRE |
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