Enhanced effects of DNA vaccine against botulinum neurotoxin serotype A by targeting antigen to dendritic cells
Autor: | Qing-Li Li, Xiao-Wei Zhou, Bo-Yang Chen, Yun-Zhou Yu, Dan-Yang Shi, Pei-Tang Huang, Xiaobin Pang, Guo Zhou, Jiansheng Lu |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Recombinant Fusion Proteins Lymphocyte Immunology Priming (immunology) Receptors Cell Surface chemical and pharmacologic phenomena Lymphocyte Activation DNA vaccination Minor Histocompatibility Antigens Mice 03 medical and health sciences 0302 clinical medicine Immune system Antigen Antigens CD Immunity Clostridium botulinum Vaccines DNA medicine Splenocyte Animals Humans Immunology and Allergy Lectins C-Type Botulinum Toxins Type A Mice Inbred BALB C biology Vaccination Botulism Dendritic Cells Virology Peptide Fragments Immunity Humoral 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Bacterial Vaccines biology.protein Female Antibody Single-Chain Antibodies |
Zdroj: | Immunology Letters. 190:118-124 |
ISSN: | 0165-2478 |
Popis: | As dendritic cells (DCs) play a critical role in priming antigen-specific immune responses, the efficacy of DNA vaccines may be enhanced by targeting the encoded antigen proteins to DCs. In this study, we constructed a DC-targeted DNA vaccine encoding the Hc domain of botulinum neurotoxin serotype A (AHc) fused with scDEC, a single-chain Fv antibody (scFv) specific for the DC-restricted antigen-uptake receptor DEC205. Intramuscular injections of mice with the DC-targeted DNA vaccine (pVAX1-scDEC-AHc) stimulated more DCs to mature than the non-targeted DNA vaccine (pVAX1-SAHc) in the splenocytes. The DC-targeted DNA vaccine could induce more DCs maturation at the site of inoculation. The DC-targeted DNA vaccine induced stronger AHc-specific humoral immune responses, lymphocyte proliferative responses and protective potency against BoNT/A in mice than did pVAX1-SAHc. Moreover, the DC-targeting DNA vaccine provided effective protection after only two inoculations. In summary, these results showed that the DC-targeted fusion DNA vaccine could generate strong immunity, indicating that maturation of DCs induced by pVAX1-scDEC-AHc may be helpful for priming and boosting immune responses. Thus, we propose that the strategy of targeting antigen to DCs in vivo via DEC205 can enhance effectively the potency of DNA vaccines against BoNTs or other pathogens in an animal model. |
Databáze: | OpenAIRE |
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