[Analysis on water-soluble components in roots of Changium smyrnioides among different populations by HPLC]
| Autor: | Liwen Yang, Bo-xing Cheng, Qiao-Sheng Guo, Changlin Wang, Tinghui Zhou |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Germplasm
Veterinary medicine education.field_of_study Plant Extracts Significant difference Population UPGMA Biology biology.organism_classification High-performance liquid chromatography Plant Roots Water soluble Complementary and alternative medicine Similarity (network science) Botany Pharmacology (medical) Changium General Pharmacology Toxicology and Pharmaceutics education Chromatography High Pressure Liquid Apiaceae |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 35(23) |
| ISSN: | 1001-5302 |
| Popis: | OBJECTIVE To analyze water-soluble components in the roots of Ch. smyrnioides among different populations that distributed in the main areas and give a reference for germplasm evaluation and quality control. METHOD Water-soluble components were extracted with the cold-soaking method and analyzed by HPLC, similarity coefficient was calculated by included angle cosine method according to relative content of major water-soluble components, and systematic relationships were constructed based on UPGMA method. RESULT There was significant difference in water-soluble components in root among population. Jiuhuashan population had the highest content of water-soluble extract. The content of water-soluble extract was below the pharmacopoeia standard in the root of Dalongshan population and Fushan population. There was significant difference in the HPLC chromatogram of water-soluble components in the root of Ch. smyrnioides from different populations, and the number of common peak was small. Similarity coefficient significantly ranged from 0.0306 to 0.9995 among 10 populations of Ch. smymrnioides. Water-soluble components in the root of Zijinshan population was the most unique, similarity coefficients were relatively small among Zijinshan population and the other seven populations except Hongshan population, and similarity coefficient was in a higher level of 0.9697 between Zijinshan population and Hongshan population. Water-soluble components were extremely similar in four populations that were Laoshan, Maoshan, Qinglongshan and Langyashan, and similarity coefficients among them were in a high level exceeded 0.99. 10 populations were divided into 3 groups according to clustering results. CONCLUSION Water-soluble components show a high diversity in the roots of Ch. smyrnioides among different populations, and can be clearly divided into 3 types. |
| Databáze: | OpenAIRE |
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