Comparative Metabolomics in Glycine max and Glycine soja under Salt Stress To Reveal the Phenotypes of Their Offspring
| Autor: | Hon-Ming Lam, Erxu Pi, Sai-Ming Ngai, Sau-Na Tsai, Yonghai Lu, Yiu Wa Kwan, Chunmei Wang, Qinglei Zhan |
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| Rok vydání: | 2013 |
| Předmět: |
Sodium Chloride
Biology Gas Chromatography-Mass Spectrometry Metabolomics Species Specificity Stress Physiological Spectroscopy Fourier Transform Infrared Monosaccharide Sugar chemistry.chemical_classification food and beverages Fabaceae Salt-Tolerant Plants General Chemistry biology.organism_classification Amino acid Plant Leaves Phenotype chemistry Biochemistry Seedlings Glycine Osmoprotectant Soybeans Glycine soja General Agricultural and Biological Sciences Functional genomics Chromatography Liquid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 61:8711-8721 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf402043m |
| Popis: | Metabolomics is developing as an important functional genomics tool for understanding plant systems' response to genetic and environmental changes. Here, we characterized the metabolic changes of cultivated soybean C08 (Glycine max L. Merr) and wild soybean W05 (Glycine soja Sieb.et Zucc.) under salt stress using MS-based metabolomics, in order to reveal the phenotypes of their eight hybrid offspring (9H0086, 9H0124, 9H0391, 9H0736, 9H0380, 9H0400, 9H0434, and 9H0590). Total small molecule extracts of soybean seedling leaves were profiled by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-Fourier transform mass spectrometry (LC-FT/MS). We found that wild soybean contained higher amounts of disaccharides, sugar alcohols, and acetylated amino acids than cultivated soybean, but with lower amounts of monosaccharides, carboxylic acids, and unsaturated fatty acids. Further investigations demonstrated that the ability of soybean to tolerate salt was mainly based on synthesis of compatible solutes, induction of reactive oxygen species (ROS) scavengers, cell membrane modifications, and induction of plant hormones. On the basis of metabolic phenotype, the salt-tolerance abilities of 9H0086, 9H0124, 9H0391, 9H0736, 9H0380, 9H0400, 9H0434, and 9H0590 were discriminated. Our results demonstrated that MS-based metabolomics provides a fast and powerful approach to discriminate the salt-tolerance characteristics of soybeans. |
| Databáze: | OpenAIRE |
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