High-density ZnO Nanowires as a Reversible Myogenic-Differentiation-Switch
| Autor: | Cesare Gargioli, Vito Errico, Claudia Fuoco, Ersilia Fornetti, Stefano Rufini, Christian Falconi, Stefano Cannata, Giovanni Saggio, Stefano Testa, Giuseppe Arrabito, Alessandro Desideri |
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| Přispěvatelé: | Errico, Vito, Arrabito, Giuseppe, Fornetti, Ersilia, Fuoco, Claudia, Testa, Stefano, Saggio, Giovanni, Rufini, Stefano, Cannata, Stefano Maria, Desideri, Alessandro, Falconi, Christian, Gargioli, Cesare |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Myogenic differentiation
Materials science Cell muscle differentiation 02 engineering and technology Muscle Development 010402 general chemistry Settore BIO/09 01 natural sciences Regenerative medicine ZnO nanowire ZnO nanowires mesoangioblasts tissue engineering Tissue engineering Myosin medicine mesoangioblast General Materials Science Progenitor cell Nanowires Zno nanowires Substrate (chemistry) Cell Differentiation 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology medicine.anatomical_structure Zinc Oxide 0210 nano-technology |
| Popis: | Mesoangioblasts are outstanding candidates for stem-cell therapy and are already being explored in clinical trials. However, a crucial challenge in regenerative medicine is the limited availability of undifferentiated myogenic progenitor cells because growth is typically accompanied by differentiation. Here reversible myogenic-differentiation switching during proliferation is achieved by functionalizing the glass substrate with high-density ZnO nanowires (NWs). Specifically, mesoangioblasts grown on ZnO NWs present a spherical viable undifferentiated cell state without lamellopodia formation during the entire observation time (8 days). Consistently, the myosin heavy chain, typically expressed in skeletal muscle tissue and differentiated myogenic progenitors, is completely absent. Remarkably, NWs do not induce any damage while they reversibly block differentiation, so that the differentiation capabilities are completely recovered upon cell removal from the NW-functionalized substrate and replating on standard culture glass. This is the first evidence of a reversible myogenic-differentiation switch that does not affect the viability. These results can be the first step toward for the in vitro growth of a large number of undifferentiated stem/progenitor cells and therefore can represent a breakthrough for cell-based therapy and tissue engineering. |
| Databáze: | OpenAIRE |
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