Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus strains isolated in a multicenter study of nursing home residents in Croatia

Autor: Ivana Mareković, Smilja Kalenić, Josip Milas, Dubravka Vuković, Ivana Roksandić Križan, Zrinka Bošnjak, Ana Budimir, Vanda Plečko, Marina Payerl Pal
Rok vydání: 2014
Předmět:
DNA
Bacterial
Methicillin-Resistant Staphylococcus aureus
Veterinary medicine
Genotype
Patients
Epidemiology
Croatia
Health Personnel
Microbial Sensitivity Tests
medicine.disease_cause
medicine
Pulsed-field gel electrophoresis
Prevalence
Humans
Typing
Staphylococcal Protein A
Genotyping
Genotyping Techniques
Molecular Epidemiology
business.industry
Health Policy
Public Health
Environmental and Occupational Health
biochemical phenomena
metabolism
and nutrition
Staphylococcal Infections
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Anti-Bacterial Agents
Nursing Homes
Molecular Typing
Nasal Mucosa
Infectious Diseases
Carriage
Cross-Sectional Studies
Staphylococcus aureus
Carrier State
business
Nursing homes
nursing home
methicillin-resistant Staphylococcus aureus
clones
Zdroj: American journal of infection control. 42(11)
ISSN: 1527-3296
Popis: Background Residents of nursing homes (NHs) are often hospitalized and could present a potential reservoir for methicillin-resistant Staphylococcus aureus (MRSA). The aim of the study was to determine the prevalence for MRSA carriage in residents and staff in Croatian NHs and to characterize MRSA strains using genotyping techniques. Methods A cross-sectional study was performed among 877 residents and staff of 7 NHs representing 3 major Croatian regions. Nasal swabs from residents and staff and other samples from residents with invasive devices were obtained. Identified isolates were submitted to susceptibility testing and genotyping with SCC mec typing, S aureus protein A (spa) locus typing, and pulsed-field gel electrophoresis (PFGE). Results The overall prevalence of MRSA colonization was 7.1% (95 confidence interval, 5.4%-8.8%), ranging from 0% to 28.8%. Four MRSA isolates were found in NH staff. All MRSA isolates were negative for Panton-Valentine leukocidin–encoding genes. SCC mec type II was found in 32 MRSA strains; SCC mec IV, in 27 strains; SCC mec I, in 3 strains. The predominant spa type was t008, found in 49 strains; PFGE analysis revealed 2 major clonal groups. Conclusions MRSA strains were found to be colonizing residents and staff of 7 NHs in Croatia. Our study demonstrates the spread of 2 clones within and among Croatian NHs. The data presented here provide an important baseline for future surveillance of MRSA in NH.
Databáze: OpenAIRE
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