Chiral Cilia Orientation in the Left-Right Organizer
| Autor: | Julien Vermot, Rita R. Ferreira, Lhéanna Klaeyle, Andrej Vilfan, Willy Supatto, Hajime Fukui, Guillaume Pakula |
|---|---|
| Přispěvatelé: | Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Optique et Biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Jozef Stefan Institute [Ljubljana] (IJS) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
SYMMETRY High Energy Physics::Lattice LEFT-RIGHT ASYMMETRY FLUID-FLOW 0601 Biochemistry and Cell Biology Quantitative Biology::Cell Behavior CENTRIOLE Rock2B 0302 clinical medicine Orientation (geometry) polycyclic compounds heterocyclic compounds spaw [SDV.BDD]Life Sciences [q-bio]/Development Biology lcsh:QH301-705.5 ComputingMilieux_MISCELLANEOUS Zebrafish media_common Physics lrdr1 0303 health sciences Cilium Dnaaf1/Lrrc50 PKD2/PC2/TRPP2/polycistin-2 DEFECTS PLANAR CELL POLARITY Classical mechanics Life Sciences & Biomedicine inorganic chemicals Quantitative Biology::Tissues and Organs media_common.quotation_subject UNCONVENTIONAL MYOSIN KUPFFERS VESICLE Asymmetry General Biochemistry Genetics and Molecular Biology MECHANISMS 03 medical and health sciences Live cell imaging MOTILITY Planar cell polarity Animals blebbistatin Cilia Symmetry breaking Body Patterning 030304 developmental biology Science & Technology Danio rerio trilobite/Vangl2 organic chemicals Organizers Embryonic technology industry and agriculture Cell Biology Zebrafish Proteins Basal Bodies tissue asymmetry Orientation (vector space) 030104 developmental biology lcsh:Biology (General) nodal Biophysics NODAL Chirality (chemistry) 030217 neurology & neurosurgery |
| Zdroj: | Cell Reports, Vol 25, Iss 8, Pp 2008-2016.e4 (2018) Cell Reports Cell Reports, Elsevier Inc, 2018, 25 (8), pp.2008-2016.e4. ⟨10.1016/j.celrep.2018.10.069⟩ 2016.e4 |
| ISSN: | 2211-1247 |
| Popis: | Summary: Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and many cell types are chiral. In the left-right organizer (LRO), cilia-driven flows transfer such chirality to the body scale. However, the existence of cellular chirality within tissues remains unknown. Here, we investigate this question in Kupffer’s vesicle (KV), the zebrafish LRO. Quantitative live imaging reveals that cilia populating the KV display asymmetric orientation between the right and left sides, resulting in a chiral structure, which is different from the chiral cilia rotation. This KV chirality establishment is dynamic and depends on planar cell polarity. While its impact on left-right (LR) symmetry breaking remains unclear, we show that this asymmetry does not depend on the LR signaling pathway or flow. This work identifies a different type of tissue asymmetry and sheds light on chirality genesis in developing tissues. : During left-right axis specification, motile cilia are required for breaking the axis of symmetry. In this context, Ferreira et al. show that cilia orientation is chiral, defining an alternative type of asymmetry in the embryo. Keywords: Danio rerio, trilobite/Vangl2, lrdr1, Dnaaf1/Lrrc50, spaw, nodal, tissue asymmetry, PKD2/PC2/TRPP2/polycistin-2, blebbistatin, Rock2B |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|