| Autor: |
Nalan Imamoglu, Pinar Atalay |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Proceedings, Vol 40, Iss 1, p 6 (2019) |
| ISSN: |
2504-3900 |
| Popis: |
Kynurenic Acid (KYNA) is a metabolite of tryptophan pathway and also an endogenousantagonist of glutamate receptors. Several studies indicated that glutamate antagonists have antiproliferativepotential. Moreover, subunits of the NMDA receptor which is one of the glutamatereceptors have been shown to be found in human hepatocellular carcinoma cell line (HepG2). Inthis study, the antitumor effects of KYNA in HepG2 cells were investigated for the first time at themolecular level. The effects of KYNA on the viability of HepG2 cells were determined by MTTanalyses. Effects of KYNA on mRNA transcriptions of apoptosis related genes Bax, Bcl-2 andCaspase-3 were analyzed by qRT-PCR. mRNA expression analysis revealed that the mRNA levelsof effector Caspase-3 and pro-apoptotic Bax/Bcl-2 ratio were not increased in HepG2 cells treatedwith KYNA. In conclusion, our findings showed that KYNA does not exert its anti-proliferativeeffects on HepG2 cells through caspase-mediated apoptotic cell death, but it may perform this antiproliferativeeffect through a different mechanism of death. Further studies are needed to find outpotential cell death mechanisms that may play a role in anti-proliferative activity of KYNA onHepG2 cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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