Enhanced Immunostimulating Activity of Lactobacilli-Mimicking Materials by Controlling Size
| Autor: | Jun Matsui, Katsuyoshi Chiba, Koji Nomoto, Kan Shida, Kaoru Moriyama, Naho Oyama, Kazuya Koumoto, Hirokazu Tsuji, Yuichi Nakagawa, Takayuki Kumano, Koji Nagahama |
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| Rok vydání: | 2015 |
| Předmět: |
Polymers
Biomedical Engineering Pharmaceutical Science Bioengineering Peptidoglycan Microbiology Mice fluids and secretions Immune system Adjuvants Immunologic Animals Cytotoxicity Cells Cultured Pharmacology Innate immune system Chemistry Macrophages Probiotics Polysaccharides Bacterial digestive oral and skin physiology Organic Chemistry food and beverages Interleukin-12 Lactobacillus Immunization Biotechnology |
| Zdroj: | Bioconjugate Chemistry. 26:1775-1781 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | The design and synthesis of materials capable of activating the immune system in a safe manner is of great interest in immunology and related fields. Lactobacilli activate the innate immune system of a host when acting as probiotics. Here, we constructed lactobacilli-mimicking materials in which polysaccharide-peptidoglycan complexes (PS-PGs) derived from lactobacilli were covalently conjugated to the surfaces of polymeric microparticles with a wide variety of sizes, ranging from 200 nm to 3 μm. The artificial lactobacilli successfully stimulated macrophages without cytotoxicity. Importantly, we found that the size of artificial lactobacilli strongly influenced their immunostimulating activities, and that artificial lactobacilli of 1 μm exhibited 10-fold higher activity than natural lactobacilli. One major advantage of the artificial lactobacilli is facile control of size, which cannot be changed in natural lactobacilli. These findings provide new insights into the design of materials for immunology as well as the molecular biology of lactobacillus. |
| Databáze: | OpenAIRE |
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