Polygenic expression of somatostatin in the sturgeonAcipenser transmontanus: Molecular cloning and distribution of the mRNAs encoding two somatostatin precursors

Autor: Robert M. Dores, Hervé Tostivint, Cristina Sollars, Michele Trabucchi, Mauro Vallarino, Isabelle Lihrmann, Hubert Vaudry
Přispěvatelé: Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Centre National de la Recherche Scientifique (CNRS), Università degli studi di Genova = University of Genoa (UniGe), University of Denver, Lihrmann, Isabelle, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Universita degli studi di Genova
Rok vydání: 2002
Předmět:
Male
Multifactorial Inheritance
MESH: Somatostatin
[SDV]Life Sciences [q-bio]
MESH: Amino Acid Sequence
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
0302 clinical medicine
Sturgeon
MESH: Animals
MESH: Brain Chemistry
Cloning
Molecular
Lungfish
0303 health sciences
General Neuroscience
Russian sturgeon
Fishes
MESH: Gene Expression Regulation
ray‐finned fish
[SDV] Life Sciences [q-bio]
Somatostatin
Acipenser transmontanus
Female
SRIF
hormones
hormone substitutes
and hormone antagonists
medicine.medical_specialty
Molecular Sequence Data
Biology
Molecular cloning
Cortistatin (neuropeptide)
03 medical and health sciences
Internal medicine
Complementary DNA
evolution
[SDV.BBM] Life Sciences [q-bio]/Biochemistry
Molecular Biology
[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
medicine
Animals
MESH: Cloning
Molecular
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Amino Acid Sequence
RNA
Messenger
MESH: RNA
Messenger
030304 developmental biology
Brain Chemistry
MESH: Molecular Sequence Data
central nervous system
biology.organism_classification
Molecular biology
MESH: Male
MESH: Fishes
Endocrinology
Gene Expression Regulation
cortistatin
MESH: Multifactorial Inheritance
MESH: Female
030217 neurology & neurosurgery
Zdroj: Journal of Comparative Neurology
Journal of Comparative Neurology, 2002, 443 (4), pp.332-345. ⟨10.1002/cne.10126⟩
Journal of Comparative Neurology, Wiley, 2002, 443 (4), pp.332-345. ⟨10.1002/cne.10126⟩
ISSN: 1096-9861
0021-9967
DOI: 10.1002/cne.10126
Popis: The sequence of somatostatin-14 (SS1) has been strongly preserved throughout the evolution of vertebrates from agnathans to mammals. In Acipenseridae (sturgeons), two isoforms of somatostatin have been characterized to date: somatostatin-14 has been identified from the gastrointestinal tract of the pallid sturgeon Scaphirhynchus albus and [Pro2]somatostatin-14 has been identified from the pituitary of the Russian sturgeon Acipenser gueldenstaedti. In the present study, we report the cloning of two distinct somatostatin cDNAs from the brain of the sturgeon Acipenser transmontanus. One of the cDNAs encodes a 116-amino acid protein (PSS1) that contains the SS1 sequence at its C-terminal extremity and, thus, is clearly orthologous to other vertebrate PSS1. The other cDNA encodes a 111-amino acid protein that contains the somatostatin variant [Pro2]somatostatin-14 at its C-terminal extremity. This second precursor exhibits more than 67% identity with the recently characterized lungfish PSS2 and goldfish PSS2. Reverse transcriptase-polymerase chain reaction analysis revealed that PSS1 is expressed in the central nervous system, the pancreas and the gut, whereas PSS2 is found in the central nervous system but not in the digestive system. In situ hybridization histochemistry showed that the PSS1 and PSS2 genes are differently expressed in numerous regions of the sturgeon brain. Interestingly, PSS1 and PSS2 mRNAs are present in the hypothalamus suggesting that, in sturgeon, both SS1 and SS2 may play hypophysiotropic functions. The PSS2 mRNA but not the PSS1 mRNA was found in the intermediate lobe of the pituitary. The present data demonstrate that two somatostatin genes are expressed in the sturgeon brain: one precursor generates somatostatin-14 and the other one gives rise to a [Pro2]somatostatin-14 variant, which is orthologous to goldfish, lungfish, and frog SS2. J. Comp. Neurol. 443:332–345, 2002. © 2002 Wiley-Liss, Inc.
Databáze: OpenAIRE
Externí odkaz: