Carbohydrate complexity and proportions of serum FSH isoforms in the male: lectin-based studies
| Autor: | C. Bergadá, Silvia Gottlieb, Verónica Ambao, S. Creus, G. Fernandez Vera, H. Benencia, Stella Campo |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Gene isoform
Adult Male endocrine system medicine.medical_specialty Aging Adolescent medicine.drug_class Population Carbohydrates Biochemistry chemistry.chemical_compound Endocrinology Internal medicine Testis medicine Concanavalin A Humans Protein Isoforms Testosterone education Child Molecular Biology Aged chemistry.chemical_classification education.field_of_study biology Puberty Lectin Biological activity Oligosaccharide Hydrogen-Ion Concentration Luteinizing Hormone Middle Aged Androgen Sertoli cell Sialic acid medicine.anatomical_structure chemistry biology.protein Follicle Stimulating Hormone Isoelectric Focusing |
| Zdroj: | Molecular and cellular endocrinology. |
| ISSN: | 0303-7207 |
| Popis: | FSH is synthesized and secreted in multiple molecular forms with different oligosaccharide structures which are needed for full expression of biological activity. GnRH and sex steroids modulate oligosaccharide structure and composition. In the present study we have assessed the carbohydrate complexity and proportion of circulating FSH isoforms during puberty, aging and after androgen administration to pubertal anorchid boys. Preparative isoelectrofocusing and lectin chromatography were used to isolate FSH isoforms on the basis of charge and internal carbohydrate complexity. Differences in sialic acid content and a progressive increase of isoforms bearing highly branched oligosaccharides were found during puberty. Less acidic, more bioactive FSH isoforms, secreted at mid-puberty may modulate important maturational events in the Sertoli cell population. Androgen administration to pubertal anorchid boys favoured the secretion of this type of isoforms. In adult men, the predominance of FSH isoforms bearing complex type oligosaccharides remained unchanged until very advanced age. These results show that the predominance of FSH isoforms bearing fully processed oligosaccharides in circulation may contribute to the development and maintenance of seminiferous epithelium function in men. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect