Krill oil extract inhibits the migration of human colorectal cancer cells and down-regulates EGFR signalling and PD-L1 expression
| Autor: | Margaret F. Veale, Xiao Q. Su, Kulmira Nurgali, Rodney B. Luwor, Abilasha Gayani Jayathilake |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
MAPK/ERK pathway PD-L1 EGFR Down-Regulation Antineoplastic Agents Apoptosis Krill oil B7-H1 Antigen Human colorectal cancer cells 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement medicine Animals Humans Epidermal growth factor receptor Protein kinase B Migration Krill oil extract biology Chemistry Cancer Cell migration lcsh:Other systems of medicine medicine.disease lcsh:RZ201-999 ErbB Receptors 030104 developmental biology Complementary and alternative medicine 030220 oncology & carcinogenesis Cancer research biology.protein Phosphorylation Colorectal Neoplasms HT29 Cells Oils Euphausiacea Research Article |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-15 (2020) BMC Complementary Medicine and Therapies |
| ISSN: | 2662-7671 |
| Popis: | Background The currently available treatments for colorectal cancer (CRC) are often associated with serious side-effects. Therefore, the development of a novel nutraceutical agent may provide an alternative complementary therapy for CRC. Overexpression of the epidermal growth factor receptor (EGFR) associates with a range of cancers while downregulation of EGFR signalling can inhibit cancer growth. Our previous studies have shown that the free fatty acid extract (FFAE) of krill oil exhibits anti-proliferative and pro-apoptotic properties. This study determines the effects of krill oil extract on the migration of human CRC cells, and its potential role in modulating EGFR signalling pathway and the expression of programmed death ligand 1 (PD-L1). Methods Human CRC cells, DLD-1 and HT-29 were treated with FFAE of KO at 0.03 and 0.12 μL/100 μL for 8 or 24 h. Cell migration was determined by Boyden chamber migration assay. The expression of EGFR, phosphorylated EGFR (pEGFR), protein kinase B (AKT), phosphorylated AKT (pAKT), extracellular signal regulated kinase (ERK1/2), phosphorylated ERK1/2 (pERK1/2) as well as PD-L1 were assessed by western blotting and immunohistochemistry. Results The FFAE of krill oil significantly inhibited cell migration compared to ethanol-treated (vehicle control) cells (P P P P P P Conclusion This study has demonstrated that krill oil may be a potential therapeutic/adjunctive agent for CRC attributed to its anti-migratory effects.. The potential anti-cancer properties of krill oil are likely to be associated with the downregulation of EGFR, pEGFR and their downstream pERK/ERK1/2 and pAKT/AKT signalling pathways along with the downregulation of PD-L1. |
| Databáze: | OpenAIRE |
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