Involvement of IGF binding protein 5 in prostaglandin E2-induced cellular senescence in human fibroblasts
| Autor: | Chansok Kim, Kwang Seok Kim, Hyo Hyun Yang, Jae-Ryong Kim, Bochan Jung |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Senescence
Aging medicine.medical_specialty medicine.medical_treatment Blotting Western Inflammation Biology Dinoprostone Internal medicine medicine Humans Prostaglandin E2 Promoter Regions Genetic Fibroblast Cells Cultured Cellular Senescence DNA Primers Skin Base Sequence Reverse Transcriptase Polymerase Chain Reaction Fibroblasts Cell biology Endocrinology medicine.anatomical_structure Eicosanoid lipids (amino acids peptides and proteins) Geriatrics and Gerontology medicine.symptom Insulin-Like Growth Factor Binding Protein 5 Gerontology Cell aging Intracellular medicine.drug Prostaglandin E |
| Zdroj: | Biogerontology. 12:239-252 |
| ISSN: | 1573-6768 1389-5729 |
| DOI: | 10.1007/s10522-010-9318-z |
| Popis: | Inflammation is an underlying basis for the molecular alterations that link aging and age-related pathological processes. In a previous study, we found that secretory phospholipase A(2) (sPLA(2)) induced cellular senescence in human dermal fibroblasts (HDFs). To further investigate the association of inflammation with cellular senescence, the effects of PGE(2) on cellular senescence in HDFs were investigated, since PGE(2) is the most abundant prostanoid. PGE(2) treatment induces cellular senescence, as determined by a decrease in cell proliferation and an increase in senescence-associated β-galactosidase staining. Notably, PGE(2) treatment increased the IGFBP5 protein level. While treatment with PGE(2) antagonists repressed PGE(2)-induced cellular senescence, increasing intracellular cAMP accelerated cellular senescence. Down-regulation of IGFBP5 inhibited PGE(2)-induced cellular senescence. Taken together, these results suggest that PGE(2) may play an important role in controlling cellular senescence of HDFs through the regulation of IGFBP5 and therefore may contribute to inflammatory disorders associated with aging. |
| Databáze: | OpenAIRE |
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