Front-Face Fluorescence Spectroscopy Allows the Characterization of Mild Heat Treatments Applied to Milk. Relations with the Denaturation of Milk Proteins
Autor: | Eric Dufour, Asylbek Kulmyrzaev, Didier Levieux |
---|---|
Přispěvatelé: | Ecole Nationale des Ingenieurs des Travaux Agricoles (ENITA), Station de recherches sur la viande, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
Rok vydání: | 2005 |
Předmět: |
Protein Denaturation
Hot Temperature Fluorophore Analytical chemistry Thermal treatment 01 natural sciences Fluorescence spectroscopy chemistry.chemical_compound MILK 0404 agricultural biotechnology [SDV.IDA]Life Sciences [q-bio]/Food engineering Animals HEAT TREATMENT Denaturation (biochemistry) Bovine serum albumin FRONT-FACE FLUORESCENCE Chromatography INTRINSIC FLUOROPHORES biology Lactoferrin MULTIVARIATE ANALYSIS 010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences General Chemistry [SDV.IDA] Life Sciences [q-bio]/Food engineering Alkaline Phosphatase Milk Proteins RADIAL IMMUNODIFFUSION 040401 food science Fluorescence 0104 chemical sciences Spectrometry Fluorescence chemistry biology.protein Alkaline phosphatase General Agricultural and Biological Sciences |
Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2005, 53 (3), pp.502-507 |
ISSN: | 1520-5118 0021-8561 |
Popis: | International audience; Emission and excitation spectra of intrinsic fluorophores present. in milk were used to evaluate changes in milk following thermal treatments in the 57-72 degreesC temperature range from 0.5 min up to 30 min. Alternatively, the concentrations of native alkaline phosphatase, lactoferrin, immunoglobulin G, bovine serum albumin, beta-lactoglobulin, and alpha-lactalbumin were determined in the same samples by enzymatic and immunochemical techniques. As principal component analysis applied to the normalized fluorescence spectra successfully discriminated different milk samples according to the temperature and time of thermal treatment, principal component regression was applied to predict the amounts of the native proteins investigated using fluorescence data. The results showed strong correlations between measured and predicted data for alkaline phosphatase and beta-lactoglobulin. This study has demonstrated that front-face fluorescence spectroscopy has a promising potential to become a rapid and nondestructive analytical technique for the evaluation of physicochemical changes in milk induced by low thermal treatment. |
Databáze: | OpenAIRE |
Externí odkaz: |