Front-Face Fluorescence Spectroscopy Allows the Characterization of Mild Heat Treatments Applied to Milk. Relations with the Denaturation of Milk Proteins

Autor: Eric Dufour, Asylbek Kulmyrzaev, Didier Levieux
Přispěvatelé: Ecole Nationale des Ingenieurs des Travaux Agricoles (ENITA), Station de recherches sur la viande, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration
Rok vydání: 2005
Předmět:
Protein Denaturation
Hot Temperature
Fluorophore
Analytical chemistry
Thermal treatment
01 natural sciences
Fluorescence spectroscopy
chemistry.chemical_compound
MILK
0404 agricultural biotechnology
[SDV.IDA]Life Sciences [q-bio]/Food engineering
Animals
HEAT TREATMENT
Denaturation (biochemistry)
Bovine serum albumin
FRONT-FACE FLUORESCENCE
Chromatography
INTRINSIC FLUOROPHORES
biology
Lactoferrin
MULTIVARIATE ANALYSIS
010401 analytical chemistry
food and beverages
04 agricultural and veterinary sciences
General Chemistry
[SDV.IDA] Life Sciences [q-bio]/Food engineering
Alkaline Phosphatase
Milk Proteins
RADIAL IMMUNODIFFUSION
040401 food science
Fluorescence
0104 chemical sciences
Spectrometry
Fluorescence
chemistry
biology.protein
Alkaline phosphatase
General Agricultural and Biological Sciences
Zdroj: Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry, American Chemical Society, 2005, 53 (3), pp.502-507
ISSN: 1520-5118
0021-8561
Popis: International audience; Emission and excitation spectra of intrinsic fluorophores present. in milk were used to evaluate changes in milk following thermal treatments in the 57-72 degreesC temperature range from 0.5 min up to 30 min. Alternatively, the concentrations of native alkaline phosphatase, lactoferrin, immunoglobulin G, bovine serum albumin, beta-lactoglobulin, and alpha-lactalbumin were determined in the same samples by enzymatic and immunochemical techniques. As principal component analysis applied to the normalized fluorescence spectra successfully discriminated different milk samples according to the temperature and time of thermal treatment, principal component regression was applied to predict the amounts of the native proteins investigated using fluorescence data. The results showed strong correlations between measured and predicted data for alkaline phosphatase and beta-lactoglobulin. This study has demonstrated that front-face fluorescence spectroscopy has a promising potential to become a rapid and nondestructive analytical technique for the evaluation of physicochemical changes in milk induced by low thermal treatment.
Databáze: OpenAIRE
Externí odkaz: