Dysfunction of the Fusion of Pre-Synaptic Plasma Membranes and Synaptic Vesicles Caused by Oxidative Stress, and its Prevention by Vitamin E
| Autor: | Hirokatsu Takatsu, Koji Fukui, Shiro Urano, Masaya Arai, Mitsunari Saito |
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| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Presynaptic Terminals Neurotransmission medicine.disease_cause Membrane Fusion Synaptic vesicle Antioxidants Internal medicine medicine TBARS Animals Vitamin E Rats Wistar Hyperoxia Chemistry General Neuroscience Cell Membrane Lipid bilayer fusion General Medicine respiratory system Rats Oxidative Stress Psychiatry and Mental health Clinical Psychology Membrane Endocrinology Biophysics Synaptic Vesicles Geriatrics and Gerontology medicine.symptom Oxidative stress |
| Zdroj: | Journal of Alzheimer's Disease. 24:759-766 |
| ISSN: | 1875-8908 1387-2877 |
| DOI: | 10.3233/jad-2011-101785 |
| Popis: | To define whether hyperoxia induces the dysfunction of membrane fusion between synaptic vesicles with pre-synaptic plasma membranes in the nerve terminals, and whether vitamin E prevents this abnormal event, we investigated the influence of hyperoxia on the fusion ability of isolated synaptic vesicles and the inside-out type pre-synaptic plasma membrane vesicles from rat brain using the fluorescence tracing method. The membrane fusion ability of both membranes from rats subjected to hyperoxia was markedly decreased compared with the membranes from a normal rat. Rats subjected to hyperoxia in the form of oxidative stress showed significant increases in the levels of thiobarbituric acid reactive substances (TBARS), conjugated dienes, and protein carbonyl moieties in both synaptic vesicles and pre-synaptic plasma membranes. When rats were supplemented with vitamin E, these abnormalities were inhibited even when rats were subjected to hyperoxia. |
| Databáze: | OpenAIRE |
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