Intracerebroventricular injection of oxidant and antioxidant molecules affects long-term potentiation in urethane anaesthetized rats
| Autor: | A Viggiano, E Viggiano, M Monda, S Ascione, S Amaro, B De Luca |
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| Přispěvatelé: | Viggiano, A, Viggiano, E, Monda, Marcellino, Ascione, S, Amaro, S, De Luca, B. |
| Předmět: |
Male
administration /&/ dosage/metabolism Xanthine Oxidase Intraventricular Long-Term Potentiation Perforant Pathway Stimulation Pharmacology Hippocampal formation Urethane Injections Rats Sprague-Dawley chemistry.chemical_compound Animals Acetylcysteine administration /&/ dosage/metabolism Analysis of Variance Anesthetics Intravenous pharmacology Animals Dentate Gyrus drug effects/physiology Electric Stimulation Excitatory Postsynaptic Potentials drug effects/physiology Free Radical Scavengers administration /&/ dosage/metabolism Hydrogen Peroxide administration /&/ dosage/metabolism Hypoxanthine administration /&/ dosage Injections Intraventricular Long-Term Potentiation drug effects/physiology Male Oxidants administration /&/ dosage/metabolism Oxidation-Reduction drug effects Perforant Pathway physiology Rats Rats Sprague-Dawley Urethane pharmacology Xanthine Oxidase administration /&/ dosage Injections Intraventricular Anesthetics chemistry.chemical_classification Reactive oxygen species Analysis of Variance Hypoxanthine Superoxide Dentate gyrus Excitatory Postsynaptic Potentials Long-term potentiation General Medicine Free Radical Scavengers Hydrogen Peroxide Oxidants Electric Stimulation Rats drug effects/physiology chemistry Biochemistry nervous system drug effects Dentate Gyrus physiology Sprague-Dawley pharmacology Tetanic stimulation Oxidation-Reduction Anesthetics Intravenous |
| Zdroj: | Scopus-Elsevier |
| Popis: | Production of superoxide anions in the incubation medium of hippocampal slices can induce long-term potentiation (LTP). Other reactive oxygen species (ROS) such as hydrogen peroxide are able to modulate LTP and are likely to be involved in aging mechanisms. The present study explored whether intracerebroventricular (ICV) injection of oxidant or antioxidant molecules could affect LTP in vivo. With this aim in mind, field excitatory post-synaptic potentials (fEPSPs) elicited by stimulation of the perforant pathway were recorded in the dentate gyrus of the hippocampal formation in urethane-anesthetized rats. N-acetyl-Lcysteine, hydrogen peroxide (H2O2) or hypoxanthine/xanthineoxidase solution (a superoxide producing system) were administrated by ICV injection. The control was represented by a group injected with saline ICV. Ten minutes after the injection, LTP was induced in the granule cells of the dentate gyrus by high frequency stimulation of the perforant pathway. Neither the H2O2 injection or the N-acetyl-L-cysteine injection caused any variation in the fEPSP at the 10-min post-injection time point, whereas the superoxide generating system caused a significant increase in the fEPSP. Moreover, at 60 min after tetanic stimulation, all treatments attenuated LTP compared with the control group. These results show that ICV administration of oxidant or antioxidant molecules can modulate LTP in vivo in the dentate gyrus. Particularly, a superoxide producing system can induce potentiation of the synaptic response. Interestingly, ICV injection of oxidants or antioxidants prevented a full expression of LTP compared to the saline injection. |
| Databáze: | OpenAIRE |
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