A colorimetric microassay for the detection of agents that interact with DNA
| Autor: | Neal Burres, Andrea Frigo, Ronald R. Rasmussen, James B. McAlpine |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pharmaceutical Science
Displacement method Analytical Chemistry Absorbance chemistry.chemical_compound Methyl Green Ethidium Drug Discovery Colorimetry Pharmacology Antibiotics Antineoplastic Organic Chemistry DNA Anti-Bacterial Agents Investigation methods Aminoglycosides Spectrometry Fluorescence Complementary and alternative medicine chemistry Biochemistry Fermentation Molecular Medicine Methyl green Ethidium bromide |
| Zdroj: | Journal of natural products. 55(11) |
| ISSN: | 0163-3864 |
| Popis: | A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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