Generation of induced pluripotent stem cell Line KAIMRCi001-A by reprogramming erythroid progenitors from peripheral blood of a healthy Saudi donor
| Autor: | Dalal Malibari, Khaled Alsayegh, Hajar Alzahrani, Kholoud Aldubayan, Mohammad Mubaraki, Moayad Baadhaim, Shereen Jamalalddin, Mustafa Daghestani, Mohammed AlBalwi, Mohammad Al-Shehri, Doaa Aboalola |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
QH301-705.5 Erythroid progenitor Induced Pluripotent Stem Cells Saudi Arabia Cell Differentiation Cell Biology General Medicine Biology Cellular Reprogramming In vitro Peripheral blood Tissue Donors Cell biology Plasmid Cell culture Humans Biology (General) Erythroid Progenitor Cells Induced pluripotent stem cell Reprogramming Developmental Biology |
| Zdroj: | Stem Cell Research, Vol 56, Iss, Pp 102548-(2021) |
| ISSN: | 1876-7753 |
| Popis: | In this study we isolated and enriched erythroid progenitor cells (EPCs) from a 10 ml peripheral blood sample from a 37-year old healthy Saudi donor. After expansion, these EPCs were reprogrammed using episomal plasmids to generate an induced pluripotent stem (iPS) cell line, KAIMRCi001-A. The pluripotency of this line was confirmed by measuring the expression of typical pluripotency markers and assessing differentiation potential in vitro. |
| Databáze: | OpenAIRE |
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