The experimental use of antisense oligonucleotides: a guide for the perplexed
| Autor: | C.A. Stein |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Genetics
Guanosine Transcription Genetic Oligonucleotide Mechanism (biology) Base pair Down-Regulation General Medicine Oligonucleotides Antisense Thionucleotides Biology Antisense RNA Transcription (biology) Antisense Technology Animals CpG Islands RNA Messenger Spotlight Gene Function (biology) Forecasting |
| Zdroj: | Journal of Clinical Investigation. 108:641-644 |
| ISSN: | 0021-9738 |
| Popis: | Antisense oligonucleotide biotechnology, at least in theory, provides a specific, rapid, and potentially high-throughput method for inhibiting gene expression and exploring gene function. Over the past decade, a plethora of experiments has been submitted, and a lesser number published, employing this approach to inhibiting gene expression at the mRNA level. The specificity of the antisense approach stems, of course, from the specificity (or, the “information content”) of the Watson-Crick base pair interaction. The fact that antisense oligonucleotides carry significant information in addition to that denoted by their nucleotide sequences is frequently overlooked. This oversight has frequently led to the lack of rigorous controls for many antisense experiments, and, on all too many occasions, to the inappropriate interpretation of experimental data (1). Indeed, serious questions have arisen as to whether an observed biological effect in an antisense experiment has indeed been produced by an antisense mechanism, or whether it is due to a complex combination of non–sequence specific effects. Investigators must therefore understand how to employ antisense technology properly and should recognize its limitations. |
| Databáze: | OpenAIRE |
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