Decreased DNA repair gene XRCC1 expression is associated with radiotherapy-induced acute side effects in breast cancer patients
| Autor: | Nuran Bese, Mehmet Güven, Gülgün S. Güven, Seda Eroz, Bahadir Batar |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
DNA Repair Genotype DNA repair DNA damage Poly ADP ribose polymerase medicine.medical_treatment Poly (ADP-Ribose) Polymerase-1 Breast Neoplasms Biology Andrology 03 medical and health sciences XRCC1 0302 clinical medicine Breast cancer PARP1 Genetics medicine Humans Genetic Predisposition to Disease Radiotherapy General Medicine medicine.disease Molecular biology Radiation therapy DNA-Binding Proteins Gene Expression Regulation Neoplastic 030104 developmental biology X-ray Repair Cross Complementing Protein 1 Gamma Rays 030220 oncology & carcinogenesis Female Poly(ADP-ribose) Polymerases Micronucleus DNA Damage |
| Zdroj: | Gene. 582(1) |
| ISSN: | 1879-0038 |
| Popis: | DNA repair plays a critical role in response to ionizing radiation (IR) and developing of radiotherapy induced normal tissue reactions. In our study, we investigated the association of radiotherapy related acute side effects, with X-ray repair cross complementing group 1 (XRCC1) and Poly (ADP-ribose) polymerase 1 (PARP1) DNA repair gene expression levels, their changes in protein expression and DNA damage levels in breast cancer patients. The study included 40 women with newly diagnosed breast cancer; an experimental case group (n=20) with acute side effects and the control group (n=20) without side effects. For gene and protein expression analysis, lymphocytes were cultured for 72 h and followed by in vitro 2 Gray (Gy) gamma-irradiation. For detection of DNA damage levels, lymphocytes were irradiated with in vitro 2 Gy gamma-rays and followed by incubation for 72 h. XRCC1 mRNA and protein expression levels were significantly higher in controls than in experimental cases (P=0.020). In terms of DNA damage levels, an increased frequency of micronucleus (MN) was observed in experimental cases versus controls, but this association was not significant (P=0.206). We also observed a significant negative correlation between MN frequency and XRCC1 protein levels in experimental (r=-0.469, P=0.037) vs control (r=-0.734, P |
| Databáze: | OpenAIRE |
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